Type

Other / n/a

Authors

David R Cotter
Toru Moriguchi
Gerard Cagney
Caitriona Scaife
Kieran Wynne
Melanie Föcking
Akiko Harauma
Jane A English

Subjects

Medicine & Nursing

Topics
medicine and health sciences mitochondrial dysfunction omega 3 schizophrenia proteomics neuritogenesis psychiatry and psychology clathrin mediated endocytosis

Omega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus. (2013)

Abstract Omega-3 fatty acid (n-3 FA) deficiency is an environmental risk factor for schizophrenia, yet characterization of the consequences of deficiency at the protein level in the brain is limited. We aimed to identify the protein pathways disrupted as a consequence of chronic n-3 deficiency in the hippocampus of mice. Fatty acid analysis of the hippocampus following chronic dietary deficiency revealed a 3-fold decrease (p < 0.001) in n-3 FA levels. Label free LC-MS/MS analysis identified and profiled 1008 proteins, of which 114 were observed to be differentially expressed between n-3 deficient and control groups (n = 8 per group). The cellular processes that were most implicated were neuritogenesis, endocytosis, and exocytosis, while specific protein pathways that were most significantly dysregulated were mitochondrial dysfunction and clathrin mediated endocytosis (CME). In order to characterize whether these processes and pathways are ones influenced by antipsychotic medication, we used LC-MS/MS to test the differential expression of these 114 proteins in the hippocampus of mice chronically treated with the antipsychotic agent haloperidol. We observed 23 of the 114 proteins to be differentially expressed, 17 of which were altered in the opposite direction to that observed following n-3 deficiency. Overall, our findings point to disturbed synaptic function, neuritogenesis, and mitochondrial function as a consequence of dietary deficiency in n-3 FA. This study greatly aids our understanding of the molecular mechanism by which n-3 deficiency impairs normal brain function, and provides clues as to how n-3 FA exert their therapeutic effect in early psychosis.
Collections Ireland -> Royal College of Surgeons in Ireland -> Psychiatry Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Psychiatry

Full list of authors on original publication

David R Cotter, Toru Moriguchi, Gerard Cagney, Caitriona Scaife, Kieran Wynne, Melanie Föcking, Akiko Harauma, Jane A English

Experts in our system

1
David R Cotter
Royal College of Surgeons in Ireland
Total Publications: 56
 
2
Gerard Cagney
Royal College of Surgeons in Ireland
Total Publications: 54
 
3
Caitriona Scaife
Royal College of Surgeons in Ireland
Total Publications: 13
 
4
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 45
 
5
Melanie Föcking
Royal College of Surgeons in Ireland
Total Publications: 20
 
6
Jane A English
Royal College of Surgeons in Ireland
Total Publications: 24