Journal Article


Caroline A Jefferies
James A Johnston
Adrien Kissenpfennig
Kevin Stacey
Barbara Coffey
Jennifer C. Byrne
Siobhán Smith
Claire Wynne
Shaun Spence
Joan Ní Gabhann



dendritic cells antigen presenting cells tlr4 il 10 btk deficient natural killer cells impaired dendritic cell induced ifn production antigen presentation

Defects in acute responses to TLR4 in Btk-deficient mice result in impaired dendritic cell-induced IFN-γ production by natural killer cells (2012)

Abstract This study defines a critical role for Btk in regulating TLR4-induced crosstalk between antigen presenting cells (APCs) and natural killer (NK) cells. Reduced levels of IL-12, IL- 18 and IFN-γ were observed in Btk-deficient mice and ex vivo generated macrophages and dendritic cells (DCs) following acute LPS administration, whilst enhanced IL-10 production was observed. In addition, upregulation of activation markers and antigen presentation molecules on APCs was also impaired in the absence of Btk. APCs, by virtue of their ability to produce IL-12 and IL-18, are strong inducers of NK-derived IFN-γ. Co-culture experiments demonstrate that Btk-deficient DCs were unable to drive wild-type or Btk-deficient NK cells to induce IFN-γ production, whereas these responses could be restored by exogenous administration of IL-12 and IL-18. Thus Btk is a critical regulator of APC-induced NK cell activation by virtue of its ability to regulate IL-12 and IL-18 production in response to acute LPS administration. © 2011 Elsevier Inc. All rights reserved.
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Full list of authors on original publication

Caroline A Jefferies, James A Johnston, Adrien Kissenpfennig, Kevin Stacey, Barbara Coffey, Jennifer C. Byrne, Siobhán Smith, Claire Wynne, Shaun Spence, Joan Ní Gabhann

Experts in our system

Caroline A Jefferies
Royal College of Surgeons in Ireland
Total Publications: 50
James A Johnston
Trinity College Dublin
Total Publications: 10
Adrien Kissenpfennig
Dublin Institute of Technology
Total Publications: 11
Siobhán Smith
Royal College of Surgeons in Ireland
Total Publications: 19
Joan Ní Gabhann
Royal College of Surgeons in Ireland
Total Publications: 23