Type

Journal Article

Authors

David Henshall
Reza Al-Aaudia
Eva Jimenez-Mateosa
Tobias Engel
Maura Heverin
Reza Al-Saudi
Steve Meaney
Eva M Jimenez-Mateos

Subjects

Psychiatry

Topics
cholesterol drug effects epilepsy kainic acid neurons oxysterol physiopathology gas chromatography mass spectrometry anti inflammatory epilepsy temporal lobe nervous system diseases chemically induced mice status epilepticus brain cholesterol lovastatin metabolism 24s hydroxycholesterol pharmacology seizures animals toxicity hippocampus brain injury

Bi-Lateral Changes to Hippocampal Cholesterol Levels During Epileptogenesis and in Chronic Epilepsy Following Focal-Onset Status Epilepticus in Mice (2012)

Abstract Brain cholesterol homeostasis has been shown to be disrupted in neurodegenerative conditions such as Alzheimer's and Huntington's diseases. Investigations in animal models of seizure-induced brain injury suggest that brain cholesterol levels are altered by prolonged seizures (status epilepticus) and are a feature of the pathophysiology of temporal lobe epilepsy. The present study measured hippocampal sterol levels in a model of unilateral hippocampal injury triggered by focal-onset status epilepticus, and in chronically epileptic mice. Status epilepticus was induced by intra-amygdala microinjection of kainic acid and ipsilateral and contralateral hippocampus analyzed. No significant changes were found for ipsilateral or contralateral hippocampal levels of desmosterol or lathosterol at any time after SE as measured by gas chromatography–mass spectrometry. 24S-hydroxycholesterol and cholesterol levels were unchanged up to 24 h after status epilepticus but were decreased in the ipsilateral hippocampus during early epileptogenesis and in chronically epileptic mice. Levels of cholesterol were also reduced in the contralateral hippocampus during epileptogenesis and in chronic epileptic mice. Treatment of mice with the anti-inflammatory cholesterol synthesis inhibitor lovastatin did not alter seizures during status epilepticus or seizure-induced neuronal death. Thus, changes to hippocampal cholesterol homeostasis predominantly begin during epileptogenesis, occur bi-laterally even when the initial precipitating injury is unilateral, and continue into the chronic epileptic period.
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Full list of authors on original publication

David Henshall, Reza Al-Aaudia, Eva Jimenez-Mateosa, Tobias Engel, Maura Heverin, Reza Al-Saudi, Steve Meaney, Eva M Jimenez-Mateos

Experts in our system

1
David C Henshall
Royal College of Surgeons in Ireland
Total Publications: 127
 
2
Tobias Engel
Royal College of Surgeons in Ireland
Total Publications: 66
 
3
Eva M Jimenez-Mateos
Royal College of Surgeons in Ireland
Total Publications: 55