Type

Journal Article

Authors

Mark Crowe
Patrick Lonergan
Niamh Forde
Marijke Eileen Beltman

Subjects

Veterinary

Topics
infertility female physiopathology embryo loss animals crosses genetic gene expression profiling rna messenger cattle diseases veterinary semen preservation anti inflammatory etiology real time polymerase chain reaction endometrium cytokines natural cytotoxicity triggering receptor 1 metabolism gene expression gene expression profiles quantitative analysis gene expression regulation pre implantation forkhead transcription factors il 6 female immunology nf kappa b p50 subunit cattle pro inflammatory pregnancy genetics cryopreservation immune response insemination artificial interleukin 6 ireland beta defensins embryo development uterus

Altered endometrial immune gene expression in beef heifers with retarded embryos (2012)

Abstract The aim of the present study was to compare endometrial gene expression profiles in a group of beef heifers yielding viable or retarded embryos on Day 7 after oestrus as a means of potentially explaining differences in embryo survival rates. Heifers were classified as either: (1) viable, when the embryo collected on Day 7 after oestrus was at the correct developmental stage (i.e. morula/early blastocyst); or (2) retarded, when the embryo was arrested at the 2–16-cell stage. The focus of the present study was on genes that were associated with either the pro- or anti-inflammatory immune response. Endometrial gene expression was determined using quantitative real-time polymerase chain reaction analysis. Expression of the β-defensin (DEFB1), interferon (IFN)-α (IFNA), IFN-γ (IFNG), interleukin (IL)-6 (IL6), IL-10 (IL10), forkhead box P3 (FOXP3) and natural cytotoxicity triggering receptor 1 (NCR1) genes was lower in endometria from viable than retarded heifers. Expression of the nuclear factor of kappa light polypeptide gene enhancer in B cells 1 (NKFB1), transforming growth factor (TGF)-β (TGFB), IFN-γ-inducible protein 16 (IFI16) and IL-21 (IL21) genes was higher in viable than retarded heifers. We propose that small disturbances in the expression of immune genes in the endometrium on Day 7 after oestrus can have detrimental effects on embryo survival.
Collections Ireland -> University College Dublin -> Veterinary Medicine Research Collection (superseded in 2015)
Ireland -> University College Dublin -> College of Agriculture, Food Science and Veterinary Medicine (superseded in 2015)
Ireland -> University College Dublin -> School of Veterinary Medicine (superseded in 2015)

Full list of authors on original publication

Mark Crowe, Patrick Lonergan, Niamh Forde, Marijke Eileen Beltman

Experts in our system

1
M A Crowe
University College Dublin
Total Publications: 101
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
N Forde
University College Dublin
Total Publications: 53
 
4
Marijke Eileen Beltman
University College Dublin
Total Publications: 24