Type

Journal Article

Authors

Mark Crowe
A. C. Evans
M. G. Diskin
M. Hilliard
G. Elia
M. P. Mullen
Marijke Eileen Beltman

Subjects

Biochemistry

Topics
histotroph beef quality platelet activating factor mass spectrometry 1 protein embryonic development embryo development proteomics

Global proteomic characterization of uterine histotroph recovered from beef heifers yielding good quality and degenerate day 7 embryos (2014)

Abstract The objective was to analyze the proteomic composition of uterine flushes collected from beef heifers on day 7 after insemination. Estrus was synchronized in crossbred beef heifers by using a protocol with a controlled intravaginal drug releasing device. Heifers detected in standing estrus (within 24-48 h after removal of controlled intravaginal drug releasing device) were inseminated (estrus = day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Heifers from which an embryo was recovered (after slaughter on day 7) were classified as either having a viable embryo (morula/blastocyst stage) or a degenerate embryo (arrested at the 2- to 16-cell stage). The overall recovery rate (viable and degenerate combined) was 64%. Global liquid chromatography coupled to tandem mass spectrometry proteomic analysis of the histotroph collected identified 40 high-confidence proteins present on day 7; 26 proteins in the viable group, 10 in the degenerate group, and 4 shared between both groups. Five proteins (platelet-activating factor acetylhydrolase IB subunit γ [PAFAH1B3], tubulin α-1D chain, tubulin β-4A chain, cytochrome C, and dihydropyrimidinase-related protein-2) were unique or more abundant in the histotroph collected from animals with a viable embryo, and 1 protein (S100-A4) was more abundant in the histotroph collected from animals with a degenerate embryo. Of interest, PAFAH1B3, detected only in histotroph from the group yielding viable embryos, belongs to the group of platelet-activating factors that are known to be important for the development of the pre-implantation embryo in other species. To our knowledge this is the first report of PAFAH1B3 in relation to bovine early embryonic development.
Collections Ireland -> University College Dublin -> Veterinary Medicine Research Collection (superseded in 2015)
Ireland -> University College Dublin -> College of Agriculture, Food Science and Veterinary Medicine (superseded in 2015)
Ireland -> University College Dublin -> School of Veterinary Medicine (superseded in 2015)

Full list of authors on original publication

Mark Crowe, A. C. Evans, M. G. Diskin, M. Hilliard, G. Elia, M. P. Mullen, Marijke Eileen Beltman

Experts in our system

1
M A Crowe
Teagasc
Total Publications: 102
 
2
A.C.O. Evans
University College Dublin
Total Publications: 93
 
3
M G Diskin
Teagasc
Total Publications: 94
 
4
Giuliano Elia
Teagasc
Total Publications: 21
 
5
Michael P. Mullen
Teagasc
Total Publications: 32
 
6
Marijke Eileen Beltman
University College Dublin
Total Publications: 24