Type

Journal Article

Authors

Stewart T. Cole
Stephen V. Gordon
Swapna Uplekar
Jeffrey M. Chen

Subjects

Microbiology

Topics
vaccines amino acid sequence polymerase chain reaction mycobacterium tuberculosis mutation mycobacterium bovis bovine tuberculosis glycine

A Point Mutation in cycA Partially Contributes to the D-cycloserine Resistance Trait of Mycobacterium bovis BCG Vaccine Strains (2012)

Abstract In mycobacteria, CycA a D-serine, L- and D-alanine, and glycine transporter also functions in the uptake of D-cycloserine, an important second-line anti-tubercular drug. A single nucleotide polymorphism identified in the cycA gene of BCG was hypothesized to contribute to the increased resistance of Mycobacterium bovis bacillus Calmette-Guérin (BCG) to D-cycloserine compared to wild-type Mycobacterium tuberculosis or Mycobacterium bovis. Working along these lines, a merodiploid strain of BCG expressing Mycobacterium tuberculosis CycA was generated and found to exhibit increased susceptibility to D-cycloserine albeit not to the same extent as wild-type Mycobacterium tuberculosis or Mycobacterium bovis. In addition, recombinant Mycobacterium smegmatis strains expressing either Mycobacterium tuberculosis or Mycobacterium bovis CycA but not BCG CycA were rendered more susceptible to D-cycloserine. These findings support the notion that CycA-mediated uptake in BCG is impaired as a result of a single nucleotide polymorphism; however, the partial contribution of this impairment to D-cycloserine resistance suggests the involvement of additional genetic lesions in this phenotype.
Collections Ireland -> University College Dublin -> Veterinary Medicine Research Collection (superseded in 2015)
Ireland -> University College Dublin -> College of Agriculture, Food Science and Veterinary Medicine (superseded in 2015)
Ireland -> University College Dublin -> School of Veterinary Medicine (superseded in 2015)

Full list of authors on original publication

Stewart T. Cole, Stephen V. Gordon, Swapna Uplekar, Jeffrey M. Chen

Experts in our system

1
Stewart T. Cole
University College Dublin
Total Publications: 3
 
2
Stephen V. Gordon
University College Dublin
Total Publications: 40