Type

Journal Article

Authors

Tara McMorrow
Peter J. Conlon
Michael P. Ryan
Gerard Cagney
Kieran Wynne
Patricia B. Maguire
William M. Gallagher
Aisling O'Riordan
Séin O'Connell
Hilary Cassidy
and 1 others

Subjects

Medicine & Nursing

Topics
biochemical markers 2 microglobulin renal transplantation blood proteins spectrometry mass matrix assisted laser desorption ionization biological markers mass spectrometry biomarkers beta 2 microglobulin roc kidney transplantation kidneys diseases seldi tof ms kidney diseases kidneys transplantation

Identification of β2-microglobulin as a urinary biomarker for chronic allograft nephropathy using proteomic methods (2011)

Abstract Chronic allograft nephropathy (CAN) remains the leading cause of renal graft loss after the first year following renal transplantation. This study aimed to identify novel urinary proteomic profiles, which could distinguish and predict CAN in susceptible individuals.Experimental Design: The study included 34 renal transplant patients with histologically proven CAN and 36 patients with normal renal transplant function. High-throughput proteomic profiles were generated from urine samples with three different ProteinChip arrays by surface-enhanced laser-desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Following SELDI a biomarker pattern software analysis was performed which led to the identification of a novel biomarker pattern that could distinguish patients with CAN from those with normal renal function.Results: An 11.7 kDa protein identified as β2 microglobulin was the primary protein of this biomarker pattern, distinguishing CAN from control patients (ROC = 0.996). SELDI-TOF-MS comparison of purified β2 microglobulin protein and CAN urine demonstrated identical 11.7 kDa protein peaks. Significantly higher concentrations of β2 microglobulin were found in the urine of patients with CAN compared to the urine of normal renal function transplant recipients (p<0.001).Conclusions and clinical relevance: Whilst further validation in a larger more diverse patient population is required to determine if this β2 microglobulin protein biomarker will provide a potential means of diagnosing CAN by non-invasive methods in a clinical setting, this study clearly shows a capability to stratify control and disease patients.
Collections Ireland -> University College Dublin -> Biomolecular and Biomedical Science Research Collection
Ireland -> University College Dublin -> Institutes and Centres
Ireland -> University College Dublin -> Conway Institute Research Collection
Ireland -> University College Dublin -> College of Science
Ireland -> University College Dublin -> Conway Institute
Ireland -> University College Dublin -> School of Biomolecular & Biomedical Science

Full list of authors on original publication

Tara McMorrow, Peter J. Conlon, Michael P. Ryan, Gerard Cagney, Kieran Wynne, Patricia B. Maguire, William M. Gallagher, Aisling O'Riordan, Séin O'Connell, Hilary Cassidy and 1 others

Experts in our system

1
Tara McMorrow
University College Dublin
Total Publications: 33
 
2
Peter J Conlon
Royal College of Surgeons in Ireland
Total Publications: 17
 
3
Michael P. Ryan
University of Limerick
Total Publications: 122
 
4
Gerard Cagney
Royal College of Surgeons in Ireland
Total Publications: 54
 
5
Kieran Wynne
Royal College of Surgeons in Ireland
Total Publications: 45
 
6
P B Maguire
University College Dublin
Total Publications: 32
 
7
William M Gallagher
University College Dublin
Total Publications: 148
 
8
Hilary Cassidy
University College Dublin
Total Publications: 10