Type

Journal Article

Authors

Christoffer Åberg
Kenneth A. Dawson
Marek W. Radomski
Maria J. Santos-Martinez
Anna Salvati
Anna Lesniak

Subjects

Chemistry

Topics
kinetics nanoparticles energy cell membrane adsorption surface properties serum free quartz crystal microbalance cellular uptake protein corona nanomaterials lipid bilayer chemistry lipid bilayers nanoparticle uptake different size cell adhesion environment cell line humans

Nanoparticle Adhesion to the Cell Membrane and Its effect on Nanoparticle Uptake Efficiency (2013)

Abstract The interactions between nanosized particles and living systems are commonly mediated by what adsorbs to the nanoparticle in the biological environment, its biomolecular corona, rather than the pristine surface. Here, we characterize the adhesion toward the cell membrane of nanoparticles of different material and size and study how this is modulated by the presence or absence of a corona on the nanoparticle surface. The results are corroborated with adsorption to simple model supported lipid bilayers using a quartz crystal microbalance. We conclude that the adsorption of proteins on the nanoparticle surface strongly reduces nanoparticle adhesion in comparison to what is observed for the bare material. Nanoparticle uptake is described as a two-step process, where the nanoparticles initially adhere to the cell membrane and subsequently are internalized by the cells via energy-dependent pathways. The lowered adhesion in the presence of proteins thereby causes a concomitant decrease in nanoparticle uptake efficiency. The presence of a biomolecular corona may confer specific interactions between the nanoparticle-corona complex and the cell surface including triggering of regulated cell uptake. An important effect of the corona is, however, a reduction in the purely unspecific interactions between the bare material and the cell membrane, which in itself disregarding specific interactions, causes a decrease in cellular uptake. We suggest that future nanoparticle-cell studies include, together with characterization of size, charge, and dispersion stability, an evaluation of the adhesion properties of the material to relevant membranes.
Collections Ireland -> University College Dublin -> School of Chemistry
Ireland -> University College Dublin -> College of Science
Ireland -> University College Dublin -> Chemistry Research Collection

Full list of authors on original publication

Christoffer Åberg, Kenneth A. Dawson, Marek W. Radomski, Maria J. Santos-Martinez, Anna Salvati, Anna Lesniak

Experts in our system

1
Christoffer Åberg
University College Dublin
Total Publications: 24
 
2
Kenneth A. Dawson
University College Dublin
Total Publications: 152
 
3
Marek W Radomski
Trinity College Dublin
Total Publications: 44
 
4
Maria Jose Santos-Martinez
Trinity College Dublin
Total Publications: 29
 
5
Anna Salvati
University College Dublin
Total Publications: 39