Journal Article


Denis C. Shields
Finian Martin
Kevin O'Brien
Norman E. Davey
Nora Khaldi
Ilias Stavropoulos



sequence alignment amino acid motifs proteomics evolution molecular conserved sequence single molecular sequence data humans disorder sequence homology amino acid cell membrane integral membrane proteins chemistry membrane potential cytoplasm intracellular membranes genetics membrane proteins membrane receptor signalling protein structure tertiary short protein folding

Protein disorder and short conserved motifs in disordered regions are enriched near the cytoplasmic side of single-pass transmembrane proteins (2012)

Abstract Intracellular juxtamembrane regions of transmembrane proteins play pivotal roles in cell signalling, mediated by protein-protein interactions. Disordered protein regions, and short conserved motifs within them, are emerging as key determinants of many such interactions. Here, we investigated whether disorder and conserved motifs are enriched in the juxtamembrane area of human single-pass transmembrane proteins. Conserved motifs were defined as short disordered regions that were much more conserved than the adjacent disordered residues. Human single-pass proteins had higher mean disorder in their cytoplasmic segments than their extracellular parts. Some, but not all, of this effect reflected the shorter length of the cytoplasmic tail. A peak of cytoplasmic disorder was seen at around 30 residues from the membrane. We noted a significant increase in the incidence of conserved motifs within the disordered regions at the same location, even after correcting for the extent of disorder. We conclude that elevated disorder within the cytoplasmic tail of many transmembrane proteins is likely to be associated with enrichment for signalling interactions mediated by conserved short motifs.
Collections Ireland -> University College Dublin -> School of Medicine
Ireland -> University College Dublin -> College of Health and Agricultural Sciences
Ireland -> University College Dublin -> Medicine Research Collection

Full list of authors on original publication

Denis C. Shields, Finian Martin, Kevin O'Brien, Norman E. Davey, Nora Khaldi, Ilias Stavropoulos

Experts in our system

Denis C. Shields
University College Dublin
Total Publications: 123
Finian Martin
University College Dublin
Total Publications: 81
Norman E. Davey
University College Dublin
Total Publications: 34