Type

Journal Article

Authors

Gerard P Sergeant
Noel G McHale
Keith D. Thornbury
Mark A Hollywood
Bernard Drumm
Sonia Kadima
Eamonn Bradley

Subjects

Pharmacology

Topics
contractile activity science smooth muscle cells muscle activity type 2 purinergic receptor materials adenosine triphosphate

Novel Excitatory Effects of Adenosine Triphosphate on Contractile and Pacemaker Activity in Rabbit Urethral Smooth Muscle (2010)

Abstract Purpose: Adenosine triphosphate is thought to be an important neurotransmitter in urethral smooth muscle but its physiological role is still unclear. We characterized the effects of adenosine triphosphate on contractile and pacemaker activity in rabbit urethral smooth muscle. Materials and Methods: Tension recordings were made from strips of rabbit proximal urethral smooth muscle. Membrane currents from freshly isolated smooth muscle cells and interstitial cells of Cajal were recorded using the patch clamp technique. Intracellular Ca 2� was measured using confocal microscopy. Results: Exogenous application of adenosine triphosphate (10 �M) evoked robust contractions that were inhibited by the type 2 purinergic receptor blocker suramin (100 �M) and the selective type 2 purinergic Y1 receptor antagonist MRS2500 (Tocris Bioscience, Ellisville, Missouri) (100 nM). Application of the type 2 purinergic Y receptor agonist 2-MeSADP (1 �M) mimicked the effects of adenosine triphosphate. When smooth muscle cells were studied under voltage clamp at �60 mV, adenosine triphosphate evoked a large single inward current (greater than 1.2 nA) but 2-MeSADP produced a small current (about 16 pA). In contrast, when interstitial cells of Cajal were held at �60 mV, they showed spontaneous transient inward currents that were increased in frequency by adenosine triphosphate and 2-MeSADP. These excitatory effects were inhibited by suramin and MRS2500. Interstitial cells of Cajal showed spontaneous Ca 2� waves that were increased in frequency by adenosine triphosphate and 2-MeSADP. These effects were also inhibited by suramin and MRS2500. Conclusions: Contractile effects of adenosine triphosphate in urethral smooth muscle are mediated by the activation of type 2 purinergic Y receptors on interstitial cells of Cajal.
Collections Ireland -> Dundalk IT -> Subject = Science
Ireland -> Dundalk IT -> Open Access DRIVERset
Ireland -> Dundalk IT -> Status = Published
Ireland -> Dundalk IT -> Type = Article

Full list of authors on original publication

Gerard P Sergeant, Noel G McHale, Keith D. Thornbury, Mark A Hollywood, Bernard Drumm, Sonia Kadima, Eamonn Bradley

Experts in our system

1
Gerard P Sergeant
Dundalk IT
Total Publications: 24
 
2
Noel G McHale
Dundalk IT
Total Publications: 35
 
3
Keith D. Thornbury
Dundalk IT
Total Publications: 27
 
4
Mark A Hollywood
Dundalk IT
Total Publications: 35
 
5
Bernard T Drumm
Royal College of Surgeons in Ireland
Total Publications: 5