Type

Journal Article

Authors

Gerard P Sergeant
Keith D. Thornbury
Noel G McHale
Mark A Hollywood
Eamonn Bradley

Subjects

Psychology

Topics
agents spontaneous science controls entry nifedipine cells calcium

Pacemaker activity in urethral interstitial cells is not dependent on capacitative calcium entry. (2005)

Abstract The aim of the present study was to investigate the properties and role of capacitative Ca(2+) entry (CCE) in interstitial cells (IC) isolated from the rabbit urethra. Ca(2+) entry in IC was larger in cells with depleted intracellular Ca(2+) stores compared with controls, consistent with influx via a CCE pathway. The nonselective Ca(2+) entry blockers Gd(3+) (10 microM), La(3+) (10 microM), and Ni(2+) (100 microM) reduced CCE by 67% (n = 14), 65% (n = 11), and 55% (n = 9), respectively. These agents did not inhibit Ca(2+) entry when stores were not depleted. Conversely, CCE in IC was resistant to SKF-96365 (10 microM), wortmannin (10 microM), and nifedipine (1 microM). Spontaneous transient inward currents were recorded from IC voltage-clamped at -60 mV. These events were not significantly affected by Gd(3+) (10 microM) or La(3+) (10 microM) and were only slightly decreased in amplitude by 100 microM Ni(2+). The results from this study demonstrate that freshly dispersed IC from the rabbit urethra possess a CCE pathway. However, influx via this pathway does not appear to contribute to spontaneous activity in these cells.
Collections Ireland -> Dundalk IT -> Subject = Science
Ireland -> Dundalk IT -> Open Access DRIVERset
Ireland -> Dundalk IT -> Status = Published
Ireland -> Dundalk IT -> Type = Article

Full list of authors on original publication

Gerard P Sergeant, Keith D. Thornbury, Noel G McHale, Mark A Hollywood, Eamonn Bradley

Experts in our system

1
Gerard P Sergeant
Dundalk IT
Total Publications: 24
 
2
Keith D. Thornbury
Dundalk IT
Total Publications: 27
 
3
Noel G McHale
Dundalk IT
Total Publications: 35
 
4
Mark A Hollywood
Dundalk IT
Total Publications: 35