Type

Journal Article

Authors

Noel G McHale
Mark A Hollywood
Gerard P Sergeant
Louise Johnston

Subjects

Pharmacology

Topics
cells ryanodine receptors oscillations science calcium urethra inositol 1 4 5 trisphosphate primary

Calcium oscillations in interstitial cells of the rabbit urethra (2005)

Abstract Measurements were made (using fast confocal microscopy) of intracellular Ca2+ levels in fluo-4 loaded interstitial cells isolated from the rabbit urethra. These cells exhibited regular Ca2+ oscillations which were associated with spontaneous transient inward currents recorded under voltage clamp. Interference with D-myo-inositol 1,4,5-trisphosphate (IP3) induced Ca2+ release using 100 microm 2-aminoethoxydiphenyl borate, and the phospholipase C (PLC) inhibitors 2-nitro-4-carboxyphenyl N,N-diphenylcarbamate and U73122 decreased the amplitude of spontaneous oscillations but did not abolish them. However, oscillations were abolished when ryanodine receptors were blocked with tetracaine or ryanodine. Oscillations ceased in the absence of external Ca2+, and frequency was directly proportional to the external Ca2+ concentration. Frequency of Ca2+ oscillation was reduced by SKF-96365, but not by nifedipine. Lanthanum and cadmium completely blocked oscillations. These results suggest that Ca2+ oscillations in isolated rabbit urethral interstitial cells are initiated by Ca2+ release from ryanodine-sensitive intracellular stores, that oscillation frequency is very sensitive to the external Ca2+ concentration and that conversion of the primary oscillation to a propagated Ca2+ wave depends upon IP3-induced Ca2+ release.
Collections Ireland -> Dundalk IT -> Subject = Science
Ireland -> Dundalk IT -> Open Access DRIVERset
Ireland -> Dundalk IT -> Status = Published
Ireland -> Dundalk IT -> Type = Article

Full list of authors on original publication

Noel G McHale, Mark A Hollywood, Gerard P Sergeant, Louise Johnston

Experts in our system

1
Noel G McHale
Dundalk IT
Total Publications: 35
 
2
Mark A Hollywood
Dundalk IT
Total Publications: 35
 
3
Gerard P Sergeant
Dundalk IT
Total Publications: 24