Type

Journal Article

Authors

Keith D. Thornbury
Noel G McHale
Gerard P Sergeant
Mark A Hollywood

Subjects

Biochemistry

Topics
cells ryanodine receptors steps inositol 1 4 5 trisphosphate receptors science activation current amphotericin b

Activation of Ca2+-activated Cl- current by depolarizing steps in rabbit urethral interstitial cells. (2003)

Abstract Interstitial cells were isolated from strips of rabbit urethra for study using the amphotericin B perforated-patch technique. Depolarizing steps to -30 mV or greater activated a Ca2+ current (ICa), followed by a Ca2+-activated Cl- current, and, on stepping back to -80 mV, large Cl- tail currents were observed. Both currents were abolished when the cells were superfused with Ca2+-free bath solution, suggesting that Ca2+ influx was necessary for activation of the Cl- current. The Cl- current was also abolished when Ba2+ was substituted for Ca2+ in the bath or the cell was dialyzed with EGTA (2 mM). The Cl- current was also reduced by cyclopiazonic acid, ryanodine, 2-aminoethoxydiphenyl borate (2-APB), and xestospongin C, suggesting that Ca2+-induced Ca2+ release (CICR) involving both ryanodine and inositol 1,4,5-trisphosphate receptors contributes to its activation.
Collections Ireland -> Dundalk IT -> Subject = Science
Ireland -> Dundalk IT -> Open Access DRIVERset
Ireland -> Dundalk IT -> Status = Published
Ireland -> Dundalk IT -> Type = Article

Full list of authors on original publication

Keith D. Thornbury, Noel G McHale, Gerard P Sergeant, Mark A Hollywood

Experts in our system

1
Keith D. Thornbury
Dundalk IT
Total Publications: 27
 
2
Noel G McHale
Dundalk IT
Total Publications: 35
 
3
Gerard P Sergeant
Dundalk IT
Total Publications: 24
 
4
Mark A Hollywood
Dundalk IT
Total Publications: 35