Type

Journal Article

Authors

Mark A Hollywood
Noel G McHale
Keith D. Thornbury
Gerard P Sergeant

Subjects

Microbiology

Topics
cells isolated phospholipase c urethra niflumic acid norepinephrine inositol 1 4 5 trisphosphate science

Characterization of norepinephrine-evoked inward currents in interstitial cells isolated from the rabbit urethra. (2002)

Abstract Freshly dispersed interstitial cells from the rabbit urethra were studied by using the perforated-patch technique. When cells were voltage clamped at -60 mV and exposed to 10 microM norepinephrine (NE) at 80-s intervals, either large single inward currents or a series of oscillatory inward currents of diminishing amplitude were evoked. These currents were blocked by either phentolamine (1 microM) or prazosin (1 microM), suggesting that the effects of NE were mediated via alpha(1)-adrenoceptors. NE-evoked currents were depressed by the blockers of Ca(2+)-activated Cl(-) currents, niflumic acid (10 microM), and 9-anthracenecarboxylic acid (9-AC, 1 mM). The reversal potential of the above currents changed in a predictable manner when the Cl(-) equilibrium potential was altered, again suggesting that they were due to activation of a Cl(-) conductance. NE-evoked currents were decreased by 10 microM cyclopiazonic acid, suggesting that they were dependent on store-released Ca(2+). Inhibition of NE-evoked currents by the phospholipase C inhibitor 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (100 microM) suggested that NE releases Ca(2+) via an inositol 1,4,5-trisphosphate (IP(3))-dependent mechanism. These results support the idea that stimulation of alpha(1)-adrenoceptors releases Ca(2+) from an IP(3)-sensitive store, which in turn activates Ca(2+)-activated Cl(-) current in freshly dispersed interstitial cells of the rabbit urethra. This elevates slow wave frequency in these cells and may underlie the mechanism responsible for increased urethral tone during nerve stimulation.
Collections Ireland -> Dundalk IT -> Subject = Science
Ireland -> Dundalk IT -> Open Access DRIVERset
Ireland -> Dundalk IT -> Status = Published
Ireland -> Dundalk IT -> Type = Article

Full list of authors on original publication

Mark A Hollywood, Noel G McHale, Keith D. Thornbury, Gerard P Sergeant

Experts in our system

1
Mark A Hollywood
Dundalk IT
Total Publications: 35
 
2
Noel G McHale
Dundalk IT
Total Publications: 35
 
3
Keith D. Thornbury
Dundalk IT
Total Publications: 27
 
4
Gerard P Sergeant
Dundalk IT
Total Publications: 24