Type

Journal Article

Authors

Sean Doyle
Mark McGee
Bernadette Earley
Eilish M Lynch

Subjects

Veterinary

Topics
holstein friesian immunophenotyping beef calves flow cytometry weaning immunology l selectin blood cell count cattle animals veterinary blood male cd4 t cells haptoglobins abrupt weaning fibrinogen abrupt weaning effects antigens surface lymphocyte subsets housing animal biology lymphocyte count

Effect of abrupt weaning at housing on leukocyte distribution, functional activity of neutrophils, and acute phase protein response of beef calves (2010)

Abstract Background: Sixteen, spring-born, single suckled, castrated male calves of Limousin × Holstein-Friesian and Simmental × Holstein-Friesian dams respectively, were used to investigate the effect of weaning on total leukocyte and differential counts, neutrophil functional activity, lymphocyte immunophenotypes, and acute phase protein response. Calves grazed with their dams until the end of the grazing season when they were housed in a slatted floor shed. On the day of housing, calves were assigned to a treatment, (i) abruptly weaned (W: n = 8) or (ii) non-weaned (controls) (C: n = 8). Weaned calves were housed in pens without their dams, whereas non-weaned (control) calves were housed with their dams. Blood was collected on day -7, 0 (housing), 2, 7, and 14 to determine total leukocyte and differential counts and concentration of fibrinogen and haptoglobin. Lymphocyte immunophenotypes were characterised using selected surface antigens (CD4+, CD8+, WC1+ (γδ T cells), MHC Class II+ lymphocytes), and the functional activities of neutrophils (surface expression of L-selectin (CD62L), phagocytic and oxidative burst activity) were investigated using flow cytometry. Results: Treatment × sampling time interactions (P < 0.05) were detected for total leukocyte and neutrophil counts, all lymphocyte subsets, mean fluorescence intensity of CD62L+ neutrophils, and percentage neutrophils performing phagocytosis. On d 2, total leukocyte and neutrophil count increased (P < 0.001), and percentage CD4+ and CD8+ lymphocytes, percentage phagocytic neutrophils, mean fluorescence intensity of CD62L+ neutrophils decreased (P < 0.05) in W compared with baseline (d 0), whereas they were unchanged (P > 0.05) in C. On d 2, percentage WC1+ lymphocytes decreased (P < 0.05), whereas percentage MHC class II+ lymphocytes increased (P < 0.05) in W and C, however the magnitude of change was greater in W than C. There were no treatment × sampling time interactions (P > 0.05) for monocyte, eosinophil, and basophil counts, percentage G1+ neutrophils, or percentage oxidative burst positive neutrophils. Conclusions: Abrupt weaning resulted in increased neutrophil counts and impaired trafficking and phagocytic function. Together with the changes in lymphocyte subsets, the results suggest that there was a greater transitory reduction in immune function at housing in abruptly weaned than non-weaned beef calves.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Teagasc -> Other Teagasc Research
Ireland -> Teagasc -> Livestock Systems
Ireland -> Teagasc -> Animal & Bioscience
Ireland -> Maynooth University -> Type = Article
Ireland -> Teagasc -> Teagasc publications in Biomed Central
Ireland -> Maynooth University -> Status = Published
Ireland -> Teagasc -> Animal & Grassland Research & Innovation Programme

Full list of authors on original publication

Sean Doyle, Mark McGee, Bernadette Earley, Eilish M Lynch

Experts in our system

1
Sean Doyle
Maynooth University
Total Publications: 130
 
2
Mark McGee
Teagasc
Total Publications: 81
 
3
Bernadette Earley
Teagasc
Total Publications: 120