Type

Journal Article

Authors

Kieran G Meade
Cliona O’Farrelly
John J Callanan
Paul Cormican
Elaine M Kenny
Derek Morris
Fernando Narciandi
Christopher J. Creevey
Aspinas Chapwanya
Cliona O'Farrelly
and 2 others

Subjects

Veterinary

Topics
molecular sequence data immunology female cattle signal transduction genetics endometrial biopsy metabolism immune response postpartum immune response dna primers base sequence beef inflammatory response next generation sequencing candidate genes real time pcr differentially expressed genes neutrophil infiltration cattle diseases uterine involution global animals high throughput nucleotide sequencing gene expression profiling veterinary transcriptomic profile t cell transcriptome real time polymerase chain reaction gene expression immunology inflammation infection endometritis gene expression regulation

Global endometrial transcriptomic profiling: transient immune activation precedes tissue proliferation and repair in healthy beef cows (2012)

Abstract Background: All cows experience bacterial contamination and tissue injury in the uterus postpartum, instigating a local inflammatory immune response. However mechanisms that control inflammation and achieve a physiologically functioning endometrium, while avoiding disease in the postpartum cow are not succinctly defined. This study aimed to identify novel candidate genes indicative of inflammation resolution during involution in healthy beef cows. Previous histological analysis of the endometrium revealed elevated inflammation 15 days postpartum (DPP) which was significantly decreased by 30 DPP. The current study generated a genome-wide transcriptomic profile of endometrial biopsies from these cows at both time points using mRNA-Seq. The pathway analysis tool GoSeq identified KEGG pathways enriched by significantly differentially expressed genes at both time points. Novel candidate genes associated with inflammatory resolution were subsequently validated in additional postpartum animals using quantitative real-time PCR (qRT-PCR). Results: mRNA-Seq revealed 1,107 significantly differentially expressed genes, 73 of which were increased 15 DPP and 1,034 were increased 30 DPP. Early postpartum, enriched immune pathways (adjusted P < 0.1) included the T cell receptor signalling pathway, graft-versus-host disease and cytokine-cytokine receptor interaction pathways. However 30 DPP, where the majority of genes were differentially expressed, the enrichment (adjusted P < 0.1) of tissue repair and proliferative activity pathways was observed. Nineteen candidate genes selected from mRNA-Seq results, were independently assessed by qRT-PCR in additional postpartum cows (5 animals) at both time points. SAA1/2, GATA2, IGF1, SHC2, and SERPINA14 genes were significantly elevated 30 DPP and are functionally associated with tissue repair and the restoration of uterine homeostasis postpartum. Conclusions: The results of this study reveal an early activation of the immune response which undergoes a temporal functional change toward tissue proliferation and regeneration during endometrial involution in healthy postpartum cows. These molecular changes mirror the activation and resolution of endometrial inflammation during involution previously classified by the degree of neutrophil infiltration. SAA1/2, GATA2, IGF1, SHC2, and SERPINA14 genes may become potential markers for resolution of endometrial inflammation in the postpartum cow.
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Full list of authors on original publication

Kieran G Meade, Cliona O’Farrelly, John J Callanan, Paul Cormican, Elaine M Kenny, Derek Morris, Fernando Narciandi, Christopher J. Creevey, Aspinas Chapwanya, Cliona O'Farrelly and 2 others

Experts in our system

1
Kieran G Meade
Teagasc
Total Publications: 50
 
2
Cliona O’Farrelly
Teagasc
Total Publications: 4
 
3
John J Callanan
University College Dublin
Total Publications: 56
 
4
Paul Cormican
Teagasc
Total Publications: 44
 
5
Elaine Kenny
Trinity College Dublin
Total Publications: 28
 
6
Derek Morris
Trinity College Dublin
Total Publications: 156
 
7
Fernando Narciandi
Teagasc
Total Publications: 11
 
8
Christopher J. Creevey
Teagasc
Total Publications: 33
 
9
Aspinas Chapwanya
Teagasc
Total Publications: 10
 
10
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 138