Type

Journal Article

Authors

Wilco C Meijer
R Paul Ross
Colin Hill
Chris Bruining
Gwenda Schaad
Paula M. O'Connor
Carmel Griffin
L Mariela Serrano
Susan Mills

Subjects

Agriculture & Food Science

Topics
listeria monocytogenes growth development mass spectrometry lactic acid nisin producing lactococci biosynthesis french artisanal cheese listeria spectrometry time factors dairy products plantaricin 423 pharmacology lactobacillus plantarum listeria innocua microbiology mass hydrogen ion concentration cheese lactobacillus plantarum lmg p 26358 food microbiology bacteriocidal activity anti bacterial agents metabolism bacteriocin nisin nisin producing culture matrix assisted laser

Inhibitory activity of Lactobacillus plantarum LMG P-26358 against Listeria innocua when used as an adjunct starter in the manufacture of cheese (2011)

Abstract Lactobacillus plantarum LMG P-26358 isolated from a soft French artisanal cheese produces a potent class IIa bacteriocin with 100% homology to plantaricin 423 and bacteriocidal activity against Listeria innocua and Listeria monocytogenes. The bacteriocin was found to be highly stable at temperatures as high as 100°C and pH ranges from 1-10. While this relatively narrow spectrum bacteriocin also exhibited antimicrobial activity against species of enterococci, it did not inhibit dairy starters including lactococci and lactobacilli when tested by well diffusion assay (WDA). In order to test the suitability of Lb. plantarum LMG P-26358 as an anti-listerial adjunct with nisin-producing lactococci, laboratory-scale cheeses were manufactured. Results indicated that combining Lb. plantarum LMG P-26358 (at 108 colony forming units (cfu)/ml) with a nisin producer is an effective strategy to eliminate the biological indicator strain, L. innocua. Moreover, industrial-scale cheeses also demonstrated that Lb. plantarum LMG P-26358 was much more effective than the nisin producer alone for protection against the indicator. MALDI-TOF mass spectrometry confirmed the presence of plantaricin 423 and nisin in the appropriate cheeses over an 18 week ripening period. A spray-dried fermentate of Lb. plantarum LMG P-26358 also demonstrated potent anti-listerial activity in vitro using L. innocua. Overall, the results suggest that Lb. plantarum LMG P-26358 is a suitable adjunct for use with nisin-producing cultures to improve the safety and quality of dairy products.
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Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Wilco C Meijer, R Paul Ross, Colin Hill, Chris Bruining, Gwenda Schaad, Paula M. O'Connor, Carmel Griffin, L Mariela Serrano, Susan Mills

Experts in our system

1
Wilco C Meijer
University College Cork
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Paula M. O'Connor
Teagasc
Total Publications: 85
 
5
Carmel Griffin
University College Cork
 
6
L Mariela Serrano
University College Cork
Total Publications: 3
 
7
Susan Mills
Teagasc
Total Publications: 15