Type

Journal Article

Authors

R Paul Ross
Colin Hill
Paul D. Cotter
Des Field
Patrick G. Casey
Alicia Campion

Subjects

Microbiology

Topics
antimicrobial food borne pathogens bacteriocin lantibiotic peptide engineering nisin mutagenesis gram positive bacteria

In vivo activity of Nisin A and Nisin V against Listeria monocytogenes in mice (2013)

Abstract Background: Lantibiotics are post-translationally modified antimicrobial peptides, of which nisin A is the most extensively studied example. Bioengineering of nisin A has resulted in the generation of derivatives with increased in vitro potency against Gram-positive bacteria. Of these, nisin V (containing a Met21Val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including Listeria monocytogenes. However, this increased potency has not been tested in vivo. Results: Here we address this issue by assessing the ability of nisin A and nisin V to control a bioluminescent strain of Listeria monocytogenes EGDe in a murine infection model. More specifically, Balb/c mice were infected via the intraperitoneal route at a dose of 1 × 105 cfu/animal and subsequently treated intraperitoneally with either nisin V, nisin A or a PBS control. Bioimaging of the mice was carried out on day 3 of the trial. Animals were then sacrificed and levels of infection were quantified in the liver and spleen. Conclusion: This analysis revealed that nisin V was more effective than Nisin A with respect to controlling infection and therefore merits further investigation with a view to potential chemotherapeutic applications.
Collections Ireland -> Teagasc -> Food Biosciences
Ireland -> University College Cork -> Microbiology
Ireland -> Teagasc -> Food Programme
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> Teagasc -> Other Teagasc Research
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> Teagasc -> Teagasc publications in Biomed Central

Full list of authors on original publication

R Paul Ross, Colin Hill, Paul D. Cotter, Des Field, Patrick G. Casey, Alicia Campion

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Des Field
Teagasc
Total Publications: 30
 
5
Patrick G. Casey
University College Cork
Total Publications: 5
 
6
Alicia Campion
University College Cork
Total Publications: 5