Type

Journal Article

Authors

R Paul Ross
Paul D. Cotter
Colin Hill
Caitriona M Guinane
Karen M. Daly
Evelyn M Clayton

Subjects

Agriculture & Food Science

Topics
food pathogen virulence factors genes genus listeria listeriosis lipi 3 gene cluster listeriolysin s monocytogenes listeria innocua genomics listeria monocytogenes strains virulence pathogenesis evolution

Atypical Listeria innocua strains possess an intact LIPI-3 (2014)

Abstract Background: Listeria monocytogenes is a food-borne pathogen which is the causative agent of listeriosis and can be divided into three evolutionary lineages I, II and III. While all strains possess the well established virulence factors associated with the Listeria pathogenicity island I (LIPI-1), lineage I strains also possess an additional pathogenicity island designated LIPI-3 which encodes listeriolysin S (LLS), a post-translationally modified cytolytic peptide. Up until now, this pathogenicity island has been identified exclusively in a subset of lineage I isolates of the pathogen Listeria monocytogenes.Results: In total 64‚ÄČL. innocua strains were screened for the presence of LIPI-3. Here we report the identification of an intact LIPI-3 in 11 isolates of L. innocua and the remnants of the cluster in several others. Significantly, we can reveal that placing the L. innocua lls genes under the control of a constitutive promoter results in a haemolytic phenotype, confirming that the cluster is capable of encoding a functional haemolysin.Conclusions: Although the presence of the LIPI-3 gene cluster is confined to lineage I isolates of L. monocytogenes, a corresponding gene cluster or its remnants have been identified in many L. innocua strains.
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Full list of authors on original publication

R Paul Ross, Paul D. Cotter, Colin Hill, Caitriona M Guinane, Karen M. Daly, Evelyn M Clayton

Experts in our system

1
R Paul Ross
Teagasc
Total Publications: 441
 
2
Paul D. Cotter
Teagasc
Total Publications: 253
 
3
Colin Hill
University College Cork
Total Publications: 351
 
4
Caitriona M Guinane
Teagasc
Total Publications: 35
 
5
Karen M. Daly
Teagasc
Total Publications: 10
 
6
Evelyn M Clayton
Teagasc
Total Publications: 7