Type

Journal Article

Authors

Colin Hill
R Paul Ross
Paul D. Cotter
Catherine Stanton
Paul W O’ Toole
Emma J. Raftis
Paula M. O'Connor
Eileen F. O'Shea

Subjects

Agriculture & Food Science

Topics
bacteriocin comparative genomic hybridization lactobacillus base sequence swine probiotic microbiology animals two component bacteriocins classification l salivarius phylogeny bacteriocins isolation purification metabolism humans bacterial proteins lactobacillus salivarius chemistry genetics amino acid sequence gastrointestinal tract molecular sequence data listeria monocytogenes sequence alignment biosynthesis salivaricin

Production of multiple bacteriocins from a single locus by gastrointestinal strains of Lactobacillus salivarius (2011)

Abstract Bacteriocins produced by Lactobacillus salivarius isolates derived from gastrointestinal origin have previously demonstrated efficacy for in vivo protection against Listeria monocytogenes infection. In this study, comparative genomic analysis was employed to investigate the intraspecies diversity of seven L. salivarius isolates of human and porcine intestinal origin, based on the genome of the well characterised bacteriocin-producing strain L. salivarius UCC118. This revealed a highly conserved megaplasmid-encoded gene cluster in these strains involved in the regulation and secretion of two-component class IIb bacteriocins. However, considerable intraspecific variation was observed in the structural genes encoding the bacteriocin peptides. These ranged from close relatives of abp118 such as salivaricin P, which differs by 2 amino acids, to completely novel bacteriocins such as salivaricin T, which is characterized in this study. Salivaricin T inhibits closely related lactobacilli and bears little homology to previously characterized salivaricins. Interestingly, the two peptides responsible for salivaricin T activity, SalTα and SalTβ, share considerable identity with the component peptides of thermophilin 13, a bacteriocin produced by Streptococcus thermophilus. Furthermore, the salivaricin locus of strain DPC6488 also encodes an additional novel one-component class IId anti-listerial bacteriocin, salivaricin L. These findings suggest a high level of redundancy in the bacteriocins that can be produced by intestinal L. salivarius isolates using the same enzymatic production and export machinery. Such diversity may contribute to their ability to dominate and compete within the complex microbiota of the mammalian gut.
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Full list of authors on original publication

Colin Hill, R Paul Ross, Paul D. Cotter, Catherine Stanton, Paul W O’ Toole, Emma J. Raftis, Paula M. O'Connor, Eileen F. O'Shea

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Catherine Stanton
Teagasc
Total Publications: 261
 
5
Paul W O’ Toole
University College Cork
Total Publications: 92
 
6
Paula M. O'Connor
Teagasc
Total Publications: 85
 
7
Eileen F. O'Shea
Teagasc
Total Publications: 10