Type

Journal Article

Authors

Martin Danaher
David Brandon
Christopher Elliot
Steven Crooks
Richard O'Kennedy
Jemma Keegan

Subjects

Chemistry

Topics
liquid chromatography tandem mass spectrometry solid phase extraction surface plasmon resonance spr biosensor liver screening assay benzimidazoles high performance liquid chromatography

Detection of benzimidazole carbamates and amino metabolites in liver by surface plasmon resonance-biosensor (2011)

Abstract Two surface plasmon resonance (SPR) biosensor screening assays were developed and validated to detect 11 benzimidazole carbamate (BZT) and four amino-benzimidazole veterinary drug residues in liver tissue. The assays used polyclonal antibodies, raised in sheep, to detect BZTs and amino-benzimidazoles. A modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method was developed to isolate benzimidazole carbamate residues. Liver samples were extracted using an acetonitrile extraction method. BZTs were purified by dispersive solid phase extraction (d-SPE) using C18 sorbent. Residues of amino-benzimidazoles were effectively cleaned-up using a simple cyclohexane defatting step. The assays were validated in accordance with the performance criteria described in 2002/657/EC. The BZT assay limit of detection was calculated to be 32 μg kg−1, the detection capability (CCβ) was determined to be 50 μg kg−1 and the mean recovery of analytes was in the range 77–132%. The amino-benzimidazole assay limit of detection was determined to be 41 μg kg−1, the CCβ was determined to be 75 μg kg−1 and analyte recovery was in the range 103–116%. Biosensor assay performance was tested by analysing liver tissue from animals treated with benzimidazole drugs and comparing the results with an ultra high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) confirmatory method. All non-compliant samples were identified using the biosensor assays.
Collections Ireland -> Teagasc -> Food Safety
Ireland -> Teagasc -> Food Programme

Full list of authors on original publication

Martin Danaher, David Brandon, Christopher Elliot, Steven Crooks, Richard O'Kennedy, Jemma Keegan

Experts in our system

1
Martin Danaher
Teagasc
Total Publications: 58
 
2
Steven Crooks
Teagasc
Total Publications: 7
 
3
R O'Kennedy
Dublin City University
Total Publications: 197