Type

Journal Article

Authors

Colin Hill
R Paul Ross
Paul D. Cotter
Orla O'Sullivan
Paula M. O'Connor
Eileen F. O'Shea

Subjects

Microbiology

Topics
chemistry isolation purification listeria monocytogenes genetics metabolism antimicrobial cationic peptides gram positive bacteria bacteriocins pharmacology bactofencin lactobacillus swine alanine transaminase probiotic animals anti bacterial agents drug resistance bacterial genome mass spectrometry matrix assisted laser l salivarius drug effects staphylococcus aureus bacteriocin molecular weight

Bactofencin A, a New Type of Cationic Bacteriocin with Unusual Immunity (2013)

Abstract Bacteriocin production is an important probiotic trait of intestinal bacteria. In this study, we identify a new type ofbacteriocin, bactofencin A, produced by a porcine intestinal isolate Lactobacillus salivarius DPC6502, and assess its potencyagainst pathogenic species including Staphylococcus aureus and Listeria monocytogenes. Genome sequencing of the bacteriocinproducer revealed bfnA, which encodes the mature and highly basic (pI 10.59), 22-amino-acid defensin-like peptide. Matrixassistedlaser desorption ionization–time of flight (MALDI-TOF) mass spectral analysis determined that bactofencin A has a molecularmass of 2,782 Da and contains two cysteine residues that form an intramolecular disulfide bond. Although an ABC transporterand transport accessory protein were also present within the bacteriocin gene cluster, a classical bacteriocin immunitygene was not detected. Interestingly, a dltB homologue was identified downstream of bfnA. DltB is usually encoded within the dltoperon of many Gram-positive bacteria. It is responsible for D-alanylation of teichoic acids in the cell wall and has previouslybeen associated with bacterial resistance to cationic antimicrobial peptides. Heterologous expression of this gene conferred bactofencinA-specific immunity on sensitive strains of L. salivarius and S. aureus (although not L. monocytogenes), establishing itsrole in bacteriocin immunity. An analysis of the distribution of bfnA revealed that it was present in four additional isolates derivedfrom porcine origin and absent from five human isolates, suggesting that its distribution is host specific. Given its novelty,we anticipate that bactofencin A represents the prototype of a new class of bacteriocins characterized as being cationic, with aDltB homologue providing a cognate immunity function.
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Full list of authors on original publication

Colin Hill, R Paul Ross, Paul D. Cotter, Orla O'Sullivan, Paula M. O'Connor, Eileen F. O'Shea

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Orla O'Sullivan
Teagasc
Total Publications: 92
 
5
Paula M. O'Connor
Teagasc
Total Publications: 85
 
6
Eileen F. O'Shea
Teagasc
Total Publications: 10