Type

Journal Article

Authors

Richard O'Kennedy
Ambrose Furey
David Brandon
Chrtistopher Elliott
Aniello Anastasio
Riona Sayers
Steven Crooks
Martin Danaher
Michelle Whelan
Jemma Keegan

Subjects

Chemistry

Topics
antibodies sheep chromatography high pressure liquid cattle chemistry liquid liquid extraction bovine milk immunology isolation purification surface plasmon resonance animals metabolism biosensing techniques anthelmintics plasmon resonance spr solid phase extraction milk benzimidazoles carbamates spr biosensors tandem mass spectrometry benzimidazole carbamates quechers benzimidazole

Benzimidazole carbamate residues in milk: Detection by Surface Plasmon Resonance-biosensor, using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method for extraction (2009)

Abstract A surface plasmon resonance (SPR) biosensor screening assay was developed and validated to detect 11 benzimidazole carbamate (BZT) veterinary drug residues in milk. The polyclonal antibody used was raised in sheep against a methyl 5(6)-[(carboxypentyl)-thio]-2-benzimidazole carbamate protein conjugate. A sample preparation procedure was developed using a modified QuEChERS method. BZT residues were extracted from milk using liquid extraction/partition with a dispersive solid phase extraction clean-up step. The assay was validated in accordance with the performance criteria described in 2002/657/EC. The limit of detection of the assay was calculated from the analysis of 20 known negative milk samples to be 2.7 μg kg−1. The detection capability (CCβ) of the assay was determined to be 5 μg kg−1 for 11 benzimidazole residues and the mean recovery of analytes was in the range 81–116%. A comparison was made between the SPR-biosensor and UPLC–MS/MS analyses of milk samples (n = 26) taken from cows treated different benzimidazole products, demonstrating the SPR-biosensor assay to be fit for purpose.
Collections Ireland -> Teagasc -> Food Safety
Ireland -> Teagasc -> Food Programme

Full list of authors on original publication

Richard O'Kennedy, Ambrose Furey, David Brandon, Chrtistopher Elliott, Aniello Anastasio, Riona Sayers, Steven Crooks, Martin Danaher, Michelle Whelan, Jemma Keegan

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
 
3
Riona Sayers
Teagasc
Total Publications: 47
 
4
Steven Crooks
Teagasc
Total Publications: 7
 
5
Martin Danaher
Teagasc
Total Publications: 58