Type

Journal Article

Authors

Kevin Kavanagh
Martin Clynes
Robert O'Connor
Michael Devereux
Malachy McCann
Paula Kinsella
Barry Coyle

Subjects

Biochemistry

Topics
biology mammalian cells oxidative stress 10 phenanthroline candida albicans cells cultured metal complexes cell membrane

Induction of apoptosis in yeast and mammalian cells by exposure to 1,10-phenanthroline metal complexes (2004)

Abstract 1,10-Phenanthroline (phen) and metal–phen complexes display fungicidal and fungiststic activity, disrupt mitochondrial function and induce oxidative stress. We have examined the effect of these drugs on the structure of yeast and mammalian cell organelles and the integrity of cellular DNA. Exposure of Candida albicans to [Mn(phen)2(mal)].2H2O or [Ag2(phen)3(mal)].2H2O (mal H2=malonic acid) resulted in DNA degradation whereas exposure to phen or [Cu(phen)2(mal)].2H2O did not. All drugs induced extensive changes to the internal structure of yeast cells including retraction of the cytoplasm, nuclear fragmentation and disruption of the mitochondrion. In the case of cultured mammalian cells [Cu(phen)2(mal)].2H2O induced apoptosis as evidenced by the ladder pattern of DNA fragments following gel electrophoresis and also the blebbing of the cell membrane. The other drugs produced non-specific DNA degradation in mammalian cells. In conclusion, phen and metal–phen complexes have the potential to induce apoptosis in fungal and mammalian cells. Given their distinct mode of action compared to conventional anti-fungal drugs, phen and metal–phen complexes may represent a novel group of anti-fungal agents for use either in combination with existing drugs or in cases where resistance to conventional drugs has emerged.
Collections Ireland -> Maynooth University -> Subject = Science & Engineering: Biology
Ireland -> Maynooth University -> Subject = Science & Engineering
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Kevin Kavanagh, Martin Clynes, Robert O'Connor, Michael Devereux, Malachy McCann, Paula Kinsella, Barry Coyle

Experts in our system

1
Kevin Kavanagh
Maynooth University
Total Publications: 139
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Robert O'Connor
Dublin City University
Total Publications: 71
 
4
Michael Devereux
Dublin Institute of Technology
Total Publications: 49
 
5
Malachy McCann
Maynooth University
Total Publications: 62