Type

Journal Article

Authors

Sean Doyle
Kevin Kavanagh
Peter Gebhardt
Robert Winkler
Claire M. Neville
Emer P. Reeves
Kathrin Reiber

Subjects

Biology

Topics
iron aspergillus fumigatus biology gene expression free expression controlled maldi tof

The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron (2005)

Abstract Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.
Collections Ireland -> Maynooth University -> Subject = Science & Engineering: Biology
Ireland -> Maynooth University -> Subject = Science & Engineering
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Sean Doyle, Kevin Kavanagh, Peter Gebhardt, Robert Winkler, Claire M. Neville, Emer P. Reeves, Kathrin Reiber

Experts in our system

1
Sean Doyle
Maynooth University
Total Publications: 130
 
2
Kevin Kavanagh
Maynooth University
Total Publications: 183
 
3
Emer P Reeves
Royal College of Surgeons in Ireland
Total Publications: 63