Type

Journal Article

Authors

Cliona O'Farrelly
Grace Mulcahy
Mario A. Fares
Andrew T. Lloyd
Tharappel James
Joanna Tierney
Susan Gaines
Rowan Higgs
David J. Lynn

Subjects

Biology

Topics
antimicrobial peptides biology characterisation novel the gene expression antimicrobial peptide genes chicken

Bioinformatic discovery and initial characterisation of nine novel antimicrobial peptide genes in the chicken (2004)

Abstract Antimicrobial peptides (AMPs) are essential components of innate immunity in a range of species fromDrosophila to humans and are generally thought to act by disrupting the membrane integrity of microbes. In order to discover novel AMPs in the chicken, we have implemented a bioinformatic approach that involves the clustering of more than 420,000 chicken expressed sequence tags (ESTs). Similarity searching of proteins' predicted to be encoded by these EST clusters for homology to known AMPs has resulted in the in silico identification of full-length sequences for seven novel gallinacins (Gal-4 to Gal-10), a novel cathelicidin and a novel liver-expressed antimicrobial peptide 2 (LEAP-2) in the chicken. Differential gene expression of these novel genes has been demonstrated across a panel of chicken tissues. An evolutionary analysis of the gallinacin family has detected sites primarily in the mature AMP that are under positive selection in these molecules. The functional implications of these results are discussed.
Collections Ireland -> Maynooth University -> Subject = Science & Engineering: Biology
Ireland -> Maynooth University -> Subject = Science & Engineering
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Cliona O'Farrelly, Grace Mulcahy, Mario A. Fares, Andrew T. Lloyd, Tharappel James, Joanna Tierney, Susan Gaines, Rowan Higgs, David J. Lynn

Experts in our system

1
Cliona O'Farrelly
Trinity College Dublin
Total Publications: 126
 
2
G Mulcahy
University College Dublin
Total Publications: 84
 
3
Mario Ali Fares
Trinity College Dublin
Total Publications: 43
 
4
Andrew T. Lloyd
Trinity College Dublin
Total Publications: 17
 
5
David J Lynn
Teagasc
Total Publications: 36