Type

Journal Article

Authors

Colin Hill
R Paul Ross
Paul D. Cotter
Mary C. Rea
Orla O'Sullivan
Kiera Murphy

Subjects

Biochemistry

Topics
computational biology anti bacterial agents s adenosylmethionine data mining phylogeny trnc bacillus thuringiensis bacterial proteins sactibiotic chemistry genetics clostridium difficile amino acid sequence thuricin cd metabolism sequence alignment genome bacterial multigene family clostridium thuricin sam protein conserved sequence bacteriocins post translational modifications trnd genes bacterial molecular sequence data sequence homology amino acid

Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters (2011)

Abstract Thuricin CD is a two-component bacteriocin produced by Bacillus thuringiensis that kills a wide range of clinically significant Clostridium difficile. This bacteriocin has recently been characterized and consists of two distinct peptides, Trnb and Trna, which both possess 3 intrapeptide sulphur to a-carbon bridges and act synergistically. Indeed, thuricin CD and subtilosin Aare the only antimicrobials known to possess these unusual structures and are known as the sactibiotics (sulplur to alpha carbon-containing antibiotics). Analysis of the thuricin CD-associated gene cluster revealed the presence of genes encoding two highly unusual SAM proteins (TrnC and TrnD) which are proposed to be responsible for these unusual post-translational modifications. On the basis of the frequently high conservation among enzymes responsible for the post-translational modification of specific antimicrobials, we performed an in silico screen for novel thuricin CD–like gene clusters using the TrnC and TrnD radical SAM proteins as driver sequences to perform an initial homology search against the complete nonredundantdatabase. Fifteen novel thuricin CD–like gene clusters were identified, based on the presence of TrnC and TrnDhomologues in the context of neighbouring genes encoding potential bacteriocin structural peptides. Moreover,metagenomic analysis revealed that TrnC or TrnD homologs are present in a variety of metagenomic environments,suggesting a widespread distribution of thuricin-like operons in a variety of environments. In-silico analysis of radical SAM proteins is sufficient to identify novel putative sactibiotic clusters.
Collections Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre
Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Colin Hill, R Paul Ross, Paul D. Cotter, Mary C. Rea, Orla O'Sullivan, Kiera Murphy

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Paul D. Cotter
Teagasc
Total Publications: 253
 
4
Mary C. Rea
Teagasc
Total Publications: 68
 
5
Orla O'Sullivan
Teagasc
Total Publications: 92
 
6
Kiera Murphy
Teagasc
Total Publications: 29