Type

Journal Article

Authors

Cormac G. Gahan
Phillip E. Klebba
Colin Hill
Richard E. Cowart
Susan A. Joyce
Roy D. Sleator
Alain Charbit
Trevor Darby
Pat G. Casey
Hualiang Pi
and 3 others

Subjects

Microbiology

Topics
haem toxicity fur ferric uptake regulator mice frva membrane protein wax moth galleria mellonella listeria monocytogenes virulence

A putative p-type ATPase required for virulence andresistance to haem toxicity in Listeria monocytogenes (2012)

Abstract Regulation of iron homeostasis in many pathogens is principally mediated by the ferric uptake regulator, Fur. Since acquisition of iron from the host is essential for the intracellular pathogen Listeria monocytogenes, we predicted the existence of Fur-regulated systems that support infection. We examined the contribution of nine Fur-regulated loci to the pathogenicity of L. monocytogenes in a murine model of infection. While mutating the majority of the genes failed to affect virulence, three mutants exhibited a significantly compromised virulence potential. Most striking was the role of the membrane protein we designate FrvA (Fur regulated virulence factor A; encoded by frvA [lmo0641]), which is absolutely required for the systemic phase of infection in mice and also for virulence in an alternative infection model, the Wax Moth Galleria mellonella. Further analysis of the DfrvA mutant revealed poor growth in iron deficient media and inhibition ofgrowth by micromolar concentrations of haem or haemoglobin, a phenotype which may contribute to the attenuated growth of this mutant during infection. Uptake studies indicated that the DfrvA mutant is unaffected in the uptake of ferriccitrate but demonstrates a significant increase in uptake of haem and haemin. The data suggest a potential role for FrvA as a haem exporter that functions, at least in part, to protect the cell against the potential toxicity of free haem.
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Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Cormac G. Gahan, Phillip E. Klebba, Colin Hill, Richard E. Cowart, Susan A. Joyce, Roy D. Sleator, Alain Charbit, Trevor Darby, Pat G. Casey, Hualiang Pi and 3 others

Experts in our system

1
Cormac G M Gahan
University College Cork
Total Publications: 109
 
2
Colin Hill
University College Cork
Total Publications: 351
 
3
Susan A. Joyce
University College Cork
Total Publications: 42
 
4
Roy D. Sleator
University College Cork
Total Publications: 44
 
5
Trevor Darby
University College Cork
Total Publications: 4
 
6
Pat G. Casey
University College Cork
Total Publications: 40