Type

Journal Article

Authors

Colin Hill
R Paul Ross
William Meaney
James Flynn
Fiona Crispie
Katja Klostermann
R Paul Ross

Subjects

Veterinary

Topics
pharmacology mastitis bovine mammary glands animal fermentation lacticin 481 animals microbiology veterinary anti bacterial agents teat dip staphylococcal infections non chemical staphylococcus aureus dairy cows dairying drug effects lactococcus lactis bacteriocins female prevention control staphylococcus lacticin 3147 cattle natural metabolism mastitis prevention

Efficacy of a teat dip containing the bacteriocin lacticin 3147 to eliminate Gram-positive pathogens associated with bovine mastitis (2009)

Abstract On most dairy farms teat dips are applied to the teats of cows either before or after milking in order to prevent pathogens from gaining access to the mammary gland via the teat canal. In the present experiments, a natural teat dip was developed using a fermentate containing the live bacterium Lactococcus lactis DPC 3251. This bacterium produces lacticin 3147, a two-component lantibiotic which was previously shown to effectively kill Gram-positive mastitis pathogens. Lacticin 3147 activity in the fermentate was retained at 53% of its original level following storage for 3 weeks at 4°C. In the initial experiments in vitro, 105 colony-forming units/ml (cfu/ml) of either Staphylococcus aureus, Streptococcus dysgalactiae or Streptococcus uberis were introduced into the lacticin-containing fermentate. Neither Staph. aureus nor Str. dysgalactiae could be detected after 30 min or 15 min, respectively, while Str. uberis was reduced approximately 100-fold after 15 min. Following these trials, preliminary experiments were performed in vivo on teats of lactating dairy cows. In these experiments, teats were coated with each of the challenge organisms and then dipped with the lacticin-containing fermented teat dip. Following a dip contact time of 10 min, staphylococci were reduced by 80% when compared with the undipped control teat. Streptococcal challenges were reduced by 97% for Str. dysgalactiae and by 90% for Str. uberis. These trials showed that the teat dip is able to reduce mastitis pathogens on the teats of lactating cows.
Collections Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre
Ireland -> University College Cork -> Microbiology - Journal Articles
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Colin Hill, R Paul Ross, William Meaney, James Flynn, Fiona Crispie, Katja Klostermann, R Paul Ross

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
James Flynn
Teagasc
Total Publications: 15
 
4
Fiona Crispie
Teagasc
Total Publications: 30