Journal Article


Alan L. Kelly
R Paul Ross
Thom Huppertz
Bernadette O'Brien
Paula O'Connor
Katharina Hinz


Agriculture & Food Science

proteomics lactation proteolysis bovine milk cheese fibrinolysin caseins milk metabolism low molecular weight electrophoresis polyacrylamide gel female chemistry food handling milk proteins cattle seasons lactation stage animals microbiology 2 de

Proteomic study of proteolysis during ripening of cheddar cheese made from milk over a lactation cycle (2012)

Abstract Milk for cheese production in Ireland is predominantly produced by pasture-fed spring-calving herds. Consequently, there are marked seasonal changes in milk composition, which arise from the interactive lactational, dietary and environmental factors. In this study, Cheddar cheese was manufactured on a laboratory scale from milk taken from a spring calving herd, over a 9-month lactation cycle between early April and early December. Plasmin activity of 6-months-old Cheddar cheese samples generally decreased over ripening time. One-dimensional urea-polyacrylamide gel electrophoresis (PAGE) of cheese samples taken after 6 months of ripening showed an extensive hydrolysis of caseins, with the fastest hydrolysis of αs1-caseins in cheeses made in August. A proteomic comparison between cheeses produced from milk taken in April, August and December showed a reduction in levels of β-casein and appearance of additional products, corresponding to low molecular weight hydrolysis products of the caseins. This study has demonstrated that a seasonal milk supply causes compositional differences in Cheddar cheese, and that proteomic tools are helpful in understanding the impact of those differences.
Collections Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Food and Nutritional Sciences - Journal Articles
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre
Ireland -> University College Cork -> Food and Nutritional Sciences
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Alan L. Kelly, R Paul Ross, Thom Huppertz, Bernadette O'Brien, Paula O'Connor, Katharina Hinz

Experts in our system

Alan L. Kelly
Total Publications: 63
R Paul Ross
Total Publications: 452
Bernadette O'Brien
Total Publications: 48
Paula M. O'Connor
Total Publications: 92