Type

Journal Article

Authors

Alan L. Kelly
R Paul Ross
Thom Huppertz
Bernadette O'Brien
Paula O'Connor
Katharina Hinz

Subjects

Agriculture & Food Science

Topics
proteomics lactation proteolysis bovine milk cheese fibrinolysin caseins milk metabolism low molecular weight electrophoresis polyacrylamide gel female chemistry food handling milk proteins cattle seasons lactation stage animals microbiology 2 de

Proteomic study of proteolysis during ripening of cheddar cheese made from milk over a lactation cycle (2012)

Abstract Milk for cheese production in Ireland is predominantly produced by pasture-fed spring-calving herds. Consequently, there are marked seasonal changes in milk composition, which arise from the interactive lactational, dietary and environmental factors. In this study, Cheddar cheese was manufactured on a laboratory scale from milk taken from a spring calving herd, over a 9-month lactation cycle between early April and early December. Plasmin activity of 6-months-old Cheddar cheese samples generally decreased over ripening time. One-dimensional urea-polyacrylamide gel electrophoresis (PAGE) of cheese samples taken after 6 months of ripening showed an extensive hydrolysis of caseins, with the fastest hydrolysis of αs1-caseins in cheeses made in August. A proteomic comparison between cheeses produced from milk taken in April, August and December showed a reduction in levels of β-casein and appearance of additional products, corresponding to low molecular weight hydrolysis products of the caseins. This study has demonstrated that a seasonal milk supply causes compositional differences in Cheddar cheese, and that proteomic tools are helpful in understanding the impact of those differences.
Collections Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Food and Nutritional Sciences - Journal Articles
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre
Ireland -> University College Cork -> Food and Nutritional Sciences
Ireland -> University College Cork -> Alimentary Pharmabiotic Centre - Journal Articles

Full list of authors on original publication

Alan L. Kelly, R Paul Ross, Thom Huppertz, Bernadette O'Brien, Paula O'Connor, Katharina Hinz

Experts in our system

1
Alan L. Kelly
Teagasc
Total Publications: 63
 
2
R Paul Ross
Teagasc
Total Publications: 452
 
3
Bernadette O'Brien
Teagasc
Total Publications: 48
 
4
Paula M. O'Connor
Teagasc
Total Publications: 92