Type

Journal Article

Authors

Richard O'Kennedy
Anthony J. Killard
Lynsey Dunne
Bernadette M. Manning
Paul P. Dillon
Stephen J. Daly

Subjects

Biochemistry

Topics
biochemistry chemistry surface plasmon resonance microbiology single chain fv biotechnology aflatoxin b1 phage display technology

Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system (2002)

Abstract The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible.
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> Subject = Physical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Centre for Sensor Research (NCSR)
Ireland -> Dublin City University -> Subject = Biological Sciences: Biotechnology
Ireland -> Dublin City University -> Subject = Biological Sciences: Microbiology
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres
Ireland -> Dublin City University -> Subject = Physical Sciences: Chemistry
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools
Ireland -> Dublin City University -> Subject = Biological Sciences
Ireland -> Dublin City University -> Subject = Biological Sciences: Biochemistry
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology

Full list of authors on original publication

Richard O'Kennedy, Anthony J. Killard, Lynsey Dunne, Bernadette M. Manning, Paul P. Dillon, Stephen J. Daly

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Anthony J. Killard
Dublin City University
Total Publications: 73