Type

Journal Article

Authors

Brett Paull
Dermot Brabazon
Ekaterina Nesterenko
David Collins

Subjects

Physics

Topics
ultraviolet uv light emitting diode ultraviolet contactless conductivity detection monolithic columns stationary phase array oven monolithic porous polymer layer capillary monoplot columns fabrication light emitting diode standard deviation mechanical engineering polymer monolithic columns

Controlled ultraviolet (UV) photoinitiated fabrication of monolithic porous layer open tubular (monoPLOT) capillary columns for chromatographic applications (2012)

Abstract An automated column fabrication technique that is based on a ultraviolet (UV) light-emitting diode (LED) array oven, and provides precisely controlled "in-capillary" ultraviolet (UV) initiated polymerization at 365 nm, is presented for the production of open tubular monolithic porous polymer layer capillary (monoPLOT) columns of varying length, inner diameter (ID), and porous layer thickness. The developed approach allows the preparation of columns of varying length, because of an automated capillary delivery approach, with precisely controlled and uniform layer thickness and monolith morphology, from controlled UV power and exposure time. The relationships between direct exposure times, intensity, and layer thickness were determined, as were the effects of capillary delivery rate (indirect exposure rate), and multiple exposures on the layer thickness and axial distribution. Layer thickness measurements were taken by scanning electron microscopy (SEM), with the longitudinal homogeneity of the stationary phase confirmed using scanning capacitively coupled contactless conductivity detection (sC(4)D). The new automated UV polymerization technique presented in this work allows the fabrication of monoPLOT columns with a very high column-to-column production reproducibility, displaying a longitudinal phase thickness variation within ±0.8% RSD (relative standard deviation).
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Engineering and Computing: School of Mechanical and Manufacturing Engineering
Ireland -> Dublin City University -> Subject = Engineering: Mechanical engineering
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Engineering and Computing
Ireland -> Dublin City University -> Subject = Engineering

Full list of authors on original publication

Brett Paull, Dermot Brabazon, Ekaterina Nesterenko, David Collins

Experts in our system

1
Brett Paull
Dublin City University
Total Publications: 120
 
2
Dermot Brabazon
Dublin City University
Total Publications: 162