Journal Article


Dermot Brabazon
Sumsun Naher
Komal Bagga
Mercedes Vazquez
Clement Higginbotham
Laura Grehan
Tanya Levingstone
Eric Moore
Patricia Fitzpatrick
Evans Chikarakara



chemistry mice inbred balb c titanium alloy tial6v4 cell viability medical physics materials pathology laser surface modification bone and bones ti 6al 4v biocompatible materials cell survival lasers osteoblasts oxazines xanthenes surface roughness cell lines physico chemical properties laser animals mice cell proliferation surface prostheses and implants titanium cell line fibroblasts alloys biomedical engineering cytology surface properties dna resazurin

In vitro fibroblast and pre-osteoblastic cellular responses on laser surface modified Ti–6Al–4V (2014)

Abstract The success of any implant, dental or orthopaedic, is driven by the interaction of implant material with the surrounding tissue. In this context, the nature of the implant surface plays a direct role in determining the long term stability as physico-chemical properties of the surface affect cellular attachment, expression of proteins, and finally osseointegration. Thus to enhance the degree of integration of the implant into the host tissue, various surface modification techniques are employed. In this work, laser surface melting of titanium alloy Ti–6Al–4V was carried out using a CO2 laser with an argon gas atmosphere. Investigations were carried out to study the influence of laser surface modification on the biocompatibility of Ti–6Al–4V alloy implant material. Surface roughness, microhardness, and phase development were recorded. Initial knowledge of these effects on biocompatibility was gained from examination of the response of fibroblast cell lines, which was followed by examination of the response of osteoblast cell lines which is relevant to the applications of this material in bone repair. Biocompatibility with these cell lines was analysed via Resazurin cell viability assay, DNA cell attachment assay, and alamarBlue metabolic activity assay. Laser treated surfaces were found to preferentially promote cell attachment, higher levels of proliferation, and enhanced bioactivity when compared to untreated control samples. These results demonstrate the tremendous potential of this laser surface melting treatment to significantly improve the biocompatibility of titanium implants in vivo.
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: Advanced Processing Technology Research Centre (APTRC)
Ireland -> Dublin City University -> Subject = Engineering: Materials
Ireland -> Dublin City University -> Subject = Engineering
Ireland -> Dublin City University -> Subject = Engineering: Biomedical engineering
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres

Full list of authors on original publication

Dermot Brabazon, Sumsun Naher, Komal Bagga, Mercedes Vazquez, Clement Higginbotham, Laura Grehan, Tanya Levingstone, Eric Moore, Patricia Fitzpatrick, Evans Chikarakara

Experts in our system

Dermot Brabazon
Dublin City University
Total Publications: 162
Sumsun Naher
Dublin City University
Total Publications: 56
Komal Bagga
Dublin City University
Total Publications: 9
Tanya J Levingstone
Royal College of Surgeons in Ireland
Total Publications: 30