Type

Conference Proceedings

Authors

Brett Paull
Dermot Brabazon
Brendan O'Connor
Roisin Thompson
Paul Clarke
Ekaterina Nesterenko
Mercedes Vazquez

Subjects

Chemistry

Topics
microfluidic devices biochemistry biosensors surface area contactless conductivity detection analytical systems micro fluidic chips microfluidics

Parallel analysis of glycoproteins using lectin-functionalised monoPLOT columns integrated in micro-fluidic chips (2011)

Abstract Glycoprotein isolation, purification and analysis is critical in the production of biologics. Therefore, there is a growing interest in new methods and techniques that allow ultra fast analytical characterisation and detection of glycoproteins and their glycoforms in the biological samples employed within the biopharmaceutical industry. In that context, micro-fluidic devices offer a great potential owing to the very fast analysis they allow, the low sample/reagent consumption, the disposability, and the ease of integration with other analytical systems, among others. In this work, a micro-fluidic device integrating porous layer open tubular columns based on dimethacrylate monoliths (monoPLOT) and functionalised with Erythrina cristagalli lectin (ECL) were used for extraction of selected glycoproteins. Polymer monoliths are very versatile materials as they can be prepared with different porosities, pore sizes, and a wide variety of functionalities using many different precursors and chemistries. The monoPLOT columns were fabricated and functionalised off-chip, and then bonded to the channel walls before final channel lamination. In order to increase the monolith surface area, immobilisation of gold nano-particles (AuNPs) was carried out prior to covalent attachment of the ECL to the AuNPs. Detection was carried out with capacitively coupled contactless conductivity detection (C4D).
Collections Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> Subject = Biological Sciences: Microfluidics
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres
Ireland -> Dublin City University -> Subject = Biological Sciences: Biosensors
Ireland -> Dublin City University -> Publication Type = Conference or Workshop Item
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools
Ireland -> Dublin City University -> Subject = Biological Sciences
Ireland -> Dublin City University -> Subject = Biological Sciences: Biochemistry
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: Irish Separation Science Cluster (ISSC)

Full list of authors on original publication

Brett Paull, Dermot Brabazon, Brendan O'Connor, Roisin Thompson, Paul Clarke, Ekaterina Nesterenko, Mercedes Vazquez

Experts in our system

1
Brett Paull
Dublin City University
Total Publications: 120
 
2
Dermot Brabazon
Dublin City University
Total Publications: 162
 
3
Brendan O'Connor
Dublin City University
Total Publications: 81
 
4
Roisin Thompson
Dublin City University
Total Publications: 20
 
5
Paul Clarke
Dublin City University
Total Publications: 28