Type

Journal Article

Authors

Martin Clynes
Paula Meleady
Michael Henry
Norma O'Donovan
Paul Dowling
Naomi Walsh

Subjects

Biochemistry

Topics
gene expression regulation neoplastic rna neoplasm cancer cell lines pancreatic cancer pathology cancer aldh1a1 protein human cell line tumor clone cells pancreatic neoplasms isolation purification neoplasm invasiveness aldehyde dehydrogenase neoplasm proteins gelsolin humans cancer invasion cell biology maldi tof rna small interfering down regulation genetics enzymology up regulation cancer cells

Aldehyde dehydrogenase 1A1 and gelsolin identified as novel invasion-modulating factors in conditioned medium of pancreatic cancer cells (2008)

Abstract Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines.
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Dublin City University -> Subject = Biological Sciences: Cell biology
Ireland -> Dublin City University -> Status = Published
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Institute for Cellular Biotechnology (NICB)
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres
Ireland -> Dublin City University -> Subject = Medical Sciences: Cancer
Ireland -> Dublin City University -> Subject = Biological Sciences
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> Subject = Medical Sciences
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering

Full list of authors on original publication

Martin Clynes, Paula Meleady, Michael Henry, Norma O'Donovan, Paul Dowling, Naomi Walsh

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Paula Meleady
Dublin City University
Total Publications: 95
 
3
Michael Henry
Dublin City University
Total Publications: 78
 
4
Norma O'Donovan
Dublin City University
Total Publications: 52
 
5
Paul Dowling
Dublin City University
Total Publications: 79
 
6
Naomi Walsh
Dublin City University
Total Publications: 18