Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8 (CM#8) inhibited invasion in vitro. 2D DIGE followed by MALDI-TOF MS analysis of CM#3 and CM#8 identified 41 proteins which were differentially regulated; 27 proteins were down-regulated and 14 proteins up-regulated in the invasion-promoting CM#3 when compared to CM#8. Western blotting analysis confirmed the down-regulated expression of gelsolin and the up-regulation of aldehyde dehydrogenase 1A1 in CM#3. Down-regulation of aldehyde dehydrogenase 1A1 in Clone #3 CM and gelsolin levels in Clone #8 CM by siRNA transfection revealed an important involvement of these proteins in promoting and inhibiting invasion in these pancreatic cancer cell lines.
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Dublin City University ->
Publication Type = Article
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Maynooth University ->
Academic Unit = Faculty of Science and Engineering: Biology
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Dublin City University ->
Subject = Biological Sciences: Cell biology
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Status = Published
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Maynooth University ->
Status = Published
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Maynooth University ->
Open Access DRIVERset
Ireland ->
Dublin City University ->
DCU Faculties and Centres = Research Initiatives and Centres: National Institute for Cellular Biotechnology (NICB)
Ireland ->
Dublin City University ->
DCU Faculties and Centres = Research Initiatives and Centres
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Dublin City University ->
Subject = Medical Sciences: Cancer
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Dublin City University ->
Subject = Biological Sciences
Ireland ->
Maynooth University ->
Type = Article
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Dublin City University ->
Subject = Medical Sciences
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Maynooth University ->
Academic Unit = Faculty of Science and Engineering
Martin Clynes,
Paula Meleady,
Michael Henry,
Norma O'Donovan,
Paul Dowling,
Naomi Walsh