Type

Journal Article

Authors

Paul Dowling
Martin Clynes
Paula Meleady
Michael Henry
Susan Kennedy
Norma O'Donovan
Naomi Walsh

Subjects

Biochemistry

Topics
cancer cell protein expression clone cells cell line basement membrane cancer proteins analysis cancer invasion pancreatic cancer

Identification of pancreatic cancer invasion-related proteins by proteomic analysis (2009)

Abstract Background – Markers of pancreatic cancer invasion were investigated in two clonal populations of the cell line, MiaPaCa-2, Clone #3 (high invasion) and Clone #8 (low invasion) using proteomic profiling of an in vitro model of pancreatic cancer. Materials and methods – Using 2D-DIGE followed by MALDI-TOF MS, two clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with high and low invasive capacities were incubated on matrigel 24 hours prior to analysis to stimulate cell-ECM contact and mimic in vivo interaction with the basement membrane. Results - Sixty proteins were identified as being differentially expressed (>1.2 fold change and p ≤ 0.05) between Clone #3 and Clone #8. Proteins found to have higher abundance levels in the highly invasive Clone #3 compared to the low invasive Clone #8 include members of the chaperone activity proteins and cytoskeleton constituents whereas metabolism-associated and catalytic proteins had lower abundance levels. Differential protein expression levels of ALDH1A1, VIM, STIP1 and KRT18 and GAPDH were confirmed by immunoblot. Using RNAi technology, STIP1 knockdown significantly reduced invasion and proliferation of the highly invasive Clone #3. Knockdown of another target, VIM by siRNA in Clone #3 cells also resulted in decreased invasion abilities of Clone #3. Elevated expression of STIP1 was observed in pancreatic tumour tissue compared to normal pancreas, whereas ALDH1A1 stained at lower levels in pancreatic tumours, as detected by immunohistochemistry. Conclusion - Identification of targets which play a role in the highly invasive phenotype of pancreatic cancer may help to understand the biological behaviour, the rapid progression of this cancer and may be of importance in the development of new therapeutic strategies for pancreatic cancer.
Collections Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Dublin City University -> Status = Published
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Institute for Cellular Biotechnology (NICB)
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres
Ireland -> Dublin City University -> Subject = Medical Sciences: Cancer
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> Subject = Medical Sciences
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering

Full list of authors on original publication

Paul Dowling, Martin Clynes, Paula Meleady, Michael Henry, Susan Kennedy, Norma O'Donovan, Naomi Walsh

Experts in our system

1
Paul Dowling
Dublin City University
Total Publications: 79
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Paula Meleady
Dublin City University
Total Publications: 95
 
4
Michael Henry
Dublin City University
Total Publications: 78
 
5
Susan Kennedy
Dublin City University
Total Publications: 37
 
6
Norma O'Donovan
Dublin City University
Total Publications: 52
 
7
Naomi Walsh
Dublin City University
Total Publications: 18