Type

Journal Article

Authors

Norma O'Donovan
John Crown
Martin Clynes
Naomi Walsh

Subjects

Biochemistry

Topics
invasion genetics cell line tumor anoikis transfection cell adhesion cancer cells cell movement rna small interfering pathology integrins pancreatic neoplasms cancer biosynthesis neoplasm invasiveness humans metabolism gene expression sirna cancer invasion cell biology pancreatic cancer blotting western

Alterations in integrin expression modulates invasion of pancreatic cancer cells (2009)

Abstract Background Factors mediating the invasion of pancreatic cancer cells through the extracellular matrix (ECM) are not fully understood. Methods In this study, sub-populations of the human pancreatic cancer cell line, MiaPaCa-2 were established which displayed differences in invasion, adhesion, anoikis, anchorage-independent growth and integrin expression. Results Clone #3 displayed higher invasion with less adhesion, while Clone #8 was less invasive with increased adhesion to ECM proteins compared to MiaPaCa-2. Clone #8 was more sensitive to anoikis than Clone #3 and MiaPaCa-2, and displayed low colony-forming efficiency in an anchorage-independent growth assay. Integrins beta 1, alpha 5 and alpha 6 were over-expressed in Clone #8. Using small interfering RNA (siRNA), integrin β1 knockdown in Clone #8 cells increased invasion through matrigel and fibronectin, increased motility, decreased adhesion and anoikis. Integrin alpha 5 and alpha 6 knockdown also resulted in increased motility, invasion through matrigel and decreased adhesion. Conclusion Our results suggest that altered expression of integrins interacting with different extracellular matrixes may play a significant role in suppressing the aggressive invasive phenotype. Analysis of these clonal populations of MiaPaCa-2 provides a model for investigations into the invasive properties of pancreatic carcinoma.
Collections Ireland -> Dublin City University -> Subject = Medical Sciences: Cancer
Ireland -> Dublin City University -> Publication Type = Article
Ireland -> Dublin City University -> Subject = Biological Sciences: Cell biology
Ireland -> Dublin City University -> Status = Published
Ireland -> Dublin City University -> Subject = Biological Sciences
Ireland -> Dublin City University -> Subject = Medical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: National Institute for Cellular Biotechnology (NICB)
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres

Full list of authors on original publication

Norma O'Donovan, John Crown, Martin Clynes, Naomi Walsh

Experts in our system

1
Norma O'Donovan
Dublin City University
Total Publications: 52
 
2
John Crown
Dublin City University
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Naomi Walsh
Dublin City University
Total Publications: 18