Type

Journal Article

Authors

Colin Clarke
Niall Barron
Martin Clynes
Clair Gallagher
Paul S. Kelly
Craig Monger

Subjects

Biochemistry

Topics
cell biology cell lines quality control high throughput next generation sequencing rna seq data analysis alternative splicing

Towards next generation CHO cell biology: Bioinformatics methods for RNA-Seq-based expression profiling. (2015)

Abstract High throughput, cost effective next generation sequencing (NGS) has enabled the publication of genome sequences for Cricetulus griseus and several Chinese hamster ovary (CHO) cell lines. RNA-Seq, the utilization of NGS technology to study the transcriptome, is expanding our understanding of the CHO cell biological system in areas ranging from the analysis of transcription start sites to the discovery of small noncoding RNAs. The analysis of RNA-Seq data, often comprised of several million short reads, presents a considerable challenge. If the CHO cell biology field is to fully exploit the potential of RNA-Seq, the development of robust data analysis pipelines is critical. In this manuscript, we outline bioinformatics approaches for the stages of a typical RNA-Seq expression profiling experiment including quality control, pre-processing, alignment and de novo transcriptome assembly. Algorithms for the analysis of mRNA and microRNA (miRNA) expression as well as methods for the detection of alternative splicing from RNA-Seq data are also presented. At this relatively early stage of Cricetulus griseus genome assembly and annotation, it is likely that a combination of isoform deconvolution and raw count based methods will provide the most complete picture of transcript expression patterns in CHO cell RNA-Seq experiments.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Colin Clarke, Niall Barron, Martin Clynes, Clair Gallagher, Paul S. Kelly, Craig Monger

Experts in our system

1
Colin Clarke
Dublin City University
Total Publications: 26
 
2
Niall Barron
Dublin City University
Total Publications: 44
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Clair Gallagher
Dublin City University
Total Publications: 12
 
5
Paul S. Kelly
Dublin City University
Total Publications: 11
 
6
Craig Monger
Dublin City University
Total Publications: 3