Type

Journal Article

Authors

Harry Holthofer
Martin Clynes
Paula Meleady
Michael Henry
Mayank Saraswat
Barry Byrne
Ilkka Miinalainen
Marjut Kreivi
Luca Musante
Alessandra Ravidà

Subjects

Biochemistry

Topics
o linked plant lectins renal replacement therapy protein glycosylation extracellular matrix diabetic nephropathy dipeptidyl peptidase iv kidney disease

Glycosylation patterns of kidney proteins differ in rat diabetic nephropathy. (2014)

Abstract Diabetic nephropathy often progresses to end-stage kidney disease and, ultimately, to renal replacement therapy. Hyperglycemia per se is expected to have a direct impact on the biosynthesis of N- and O-linked glycoproteins. This study aims to establish the link between protein glycosylation and progression of experimental diabetic kidney disease using orthogonal methods. Kidneys of streptozotocin-diabetic and control rats were harvested at three different time points post streptozotocin injection. A panel of 12 plant lectins was used in the screening of lectin blots. The lectins UEAI, PHA-E, GSI, PNA, and RCA identified remarkable disease-associated differences in glycoprotein expression. Lectin affinity chromatography followed by mass spectrometric analyses led to the identification of several glycoproteins involved in salt-handling, angiogenesis, and extracellular matrix degradation. Our data confirm a substantial link between glycosylation signature and diabetes progression. Furthermore, as suggested by our findings on dipeptidyl peptidase-IV, altered protein glycosylation may reflect changes in biochemical properties such as enzymatic activity. Thus, our study demonstrates the unexplored potential of protein glycosylation analysis in the discovery of molecules linked to diabetic kidney disease.Kidney International advance online publication, 14 January 2015; doi:10.1038/ki.2014.387.
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Full list of authors on original publication

Harry Holthofer, Martin Clynes, Paula Meleady, Michael Henry, Mayank Saraswat, Barry Byrne, Ilkka Miinalainen, Marjut Kreivi, Luca Musante, Alessandra Ravidà

Experts in our system

1
Harry Holthofer
Dublin City University
Total Publications: 16
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Paula Meleady
Dublin City University
Total Publications: 95
 
4
Michael Henry
Dublin City University
Total Publications: 78
 
5
Barry Byrne
Dublin City University
Total Publications: 15
 
6
Luca Musante
Dublin City University
Total Publications: 12