Type

Journal Article

Authors

Martin Clynes
Ross Morgan
Emmet O'Brien
Michael Moriarty
John Crown
Jo Ballot
Vincent Lynch
Martin P Barr
Kenneth O'Byrne
Kathy Gately
and 5 others

Subjects

Medicine & Nursing

Topics
mass spectrometry early diagnosis cell lines cancer patients lung cancer cancer tissue two dimensional difference gel electrophoresis pyruvate kinase

Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer. (2014)

Abstract Lung cancer is the second most common type of cancer in the world and is the most common cause of cancer-related death in both men and women. Research into causes, prevention and treatment of lung cancer is ongoing and much progress has been made recently in these areas, however survival rates have not significantly improved. Therefore, it is essential to develop biomarkers for early diagnosis of lung cancer, prediction of metastasis and evaluation of treatment efficiency, as well as using these molecules to provide some understanding about tumour biology and translate highly promising findings in basic science research to clinical application. In this investigation, two-dimensional difference gel electrophoresis and mass spectrometry were initially used to analyse conditioned media from a panel of lung cancer and normal bronchial epithelial cell lines. Significant proteins were identified with heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1), pyruvate kinase M2 isoform (PKM2), Hsc-70 interacting protein and lactate dehydrogenase A (LDHA) selected for analysis in serum from healthy individuals and lung cancer patients. hnRNPA2B1, PKM2 and LDHA were found to be statistically significant in all comparisons. Tissue analysis and knockdown of hnRNPA2B1 using siRNA subsequently demonstrated both the overexpression and potential role for this molecule in lung tumorigenesis. The data presented highlights a number of in vitro derived candidate biomarkers subsequently verified in patient samples and also provides some insight into their roles in the complex intracellular mechanisms associated with tumour progression.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Martin Clynes, Ross Morgan, Emmet O'Brien, Michael Moriarty, John Crown, Jo Ballot, Vincent Lynch, Martin P Barr, Kenneth O'Byrne, Kathy Gately and 5 others

Experts in our system

1
Martin Clynes
Dublin City University
Total Publications: 209
 
2
Michael Moriarty
Dublin City University
Total Publications: 8
 
3
John Crown
Dublin City University
 
4
Vincent Lynch
Dublin City University
Total Publications: 10
 
5
Martin P Barr
Trinity College Dublin
Total Publications: 27
 
6
Kenneth J O'Byrne
Trinity College Dublin
Total Publications: 36
 
7
Kathy Gately
Trinity College Dublin
Total Publications: 19