Type

Journal Article

Authors

Richard J O'Kennedy
Christopher T Elliott
Shauna Devlin
Greg Husar
Michael J Lochhead
Charles Greef
Barry Byrne
Daniel A McPartlin
Svetlana Krivelo
Edwina Stack
and 1 others

Subjects

Engineering

Topics
novel the optical antibody affinity detection waveguide rapid detection recombinant surface plasmon resonance

Detection of the cyanobacterial toxin, microcystin-LR, using a novel recombinant antibody-based optical-planar waveguide platform. (2014)

Abstract Microcystins are a major group of cyanobacterial heptapeptide toxins found in freshwater and brackish environments. There is currently an urgent requirement for highly-sensitive, rapid and in-expensive detection methodologies for these toxins. A novel single chain fragment variable (scFv) fragment was generated and is the first known report of a recombinant anti-microcystin avian antibody. In a surface plasmon resonance-based immunoassay, the antibody fragment displayed cross-reactivity with seven microcystin congeners (microcystin-leucine-arginine (MC-LR) 100%, microcystin-tyrosine-arginine (MC-YR) 79.7%, microcystin-leucine-alanine (MC-LA) 74.8%, microcystin-leucine-phenylalanine (MC-LF) 67.5%, microcystin-leucine-tryptophan (MC-LW) 63.7%, microcystin-arginine-arginine (MC-RR) 60.1% and nodularin (Nod) 69.3%, % cross reactivity). Following directed molecular evolution of the parental clone the resultant affinity-enhanced antibody fragment was applied in an optimized fluorescence immunoassay on a planar waveguide detection system. This novel immuno-sensing format can detect free microcystin-LR with a functional limit of detection of 0.19 ng mL(-1)and a detection range of 0.21-5.9 ng mL(-1). The assay is highly reproducible (displaying percentage coefficients of variance below 8% for intra-day assays and below 11% for inter-day assays), utilizes an inexpensive cartridge system with low reagent volumes and can be completed in less than twenty minutes.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard J O'Kennedy, Christopher T Elliott, Shauna Devlin, Greg Husar, Michael J Lochhead, Charles Greef, Barry Byrne, Daniel A McPartlin, Svetlana Krivelo, Edwina Stack and 1 others

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Barry Byrne
Dublin City University
Total Publications: 15
 
3
Daniel A McPartlin
Dublin City University