Type

Journal Article

Authors

Kevin Kavanagh
Finbarr O'Sullivan
Martin Clynes
Niamh O'Reilly
Clair Gallagher
Frederick W McMahon

Subjects

Microbiology

Topics
epithelium corneal inflammatory cytokines cell line pathology il 4 blotting western humans bacterial proteins eye infections bacterial corneal ulcer il 6 cell cycle human cells inflammatory response microbiology epithelial cells enzyme linked immunosorbent assay metabolism bacillus isolation purification cell proliferation

Exposure of a corneal epithelial cell line (hTCEpi) to Demodex-associated Bacillus proteins results in an inflammatory response. (2014)

Abstract A role for a bacterium, Bacillus oleronius, originally isolated from a Demodex mite, in the induction of ocular rosacea has been proposed. The aim of this work was to characterize the response of a corneal epithelial cell line to Bacillus proteins, as this might give an insight into how such proteins contribute to the symptoms of ocular rosacea in vivo. The effect of exposing Bacillus protein preparation on human telomerase-immortalized corneal epithelial cells (hTCEpi) was measured by monitoring changes in cell proliferation and the expression of a number of genes associated with inflammation. The production of inflammatory cytokines was measured and the expression and activity of MMP-9 was quantified. Exposure of hTCEpi cells to 2 or 6 μg/mL Bacillus protein resulted in a dose-dependent reduction in cell proliferation. Exposure of cells to 6 μg/mL Bacillus protein did not induce apoptosis, but there was an increase in the expression of genes coding for IL-6 (13.8-fold), IL-1β (4.0-fold), IL-8 (11.1-fold), and TNF-α (4.1-fold). Increased expression of genes coding for the defensins, CCL20 (4.5-fold) and S100A7 (6.8-fold) also was observed. Elevated production of IL-6 and IL-8 was evident from cells exposed to 2 and 6 μg/mL Bacillus protein. The hTCEpi cells demonstrated increased MMP-9 expression (3.2-fold, P = 0.003) and activity (2.2-fold, P = 0.0186) after 48 hours of exposure to 6 μg/mL Bacillus protein preparation. The results suggest that interaction of Demodex-associated Bacillus proteins with the corneal surface could lead to tissue degradation and inflammation, possibly leading to corneal scarring.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Type = Article
Ireland -> Dublin City University -> PubMed
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Kevin Kavanagh, Finbarr O'Sullivan, Martin Clynes, Niamh O'Reilly, Clair Gallagher, Frederick W McMahon

Experts in our system

1
Kevin Kavanagh
Maynooth University
Total Publications: 139
 
2
Finbarr O'Sullivan
Dublin City University
Total Publications: 21
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Clair Gallagher
Dublin City University
Total Publications: 12