Type

Journal Article

Authors

Finbarr O'Sullivan
William Power
Martin Clynes
Conor Murphy
Andra Bobart-Hone
Sandra Shaw
Vincent Lynch
Padraig Doolan
Kishore R Katikireddy
Sinéad T Aherne
and 2 others

Subjects

Biochemistry

Topics
physiology metabolism humans corneal transplantation amnion rna mitochondrial limbus corneae gene expression profiling microarray analysis cytology cells cultured biological markers epithelium corneal rna cadaver wound healing

Comparative transcriptomic analysis of cultivated limbal epithelium and donor corneal tissue reveals altered wound healing gene expression. (2014)

Abstract The improved surgical outcomes associated with transplantation of cultivated amniotic membrane expanded limbal epithelium (AMLE) compared to traditional donor methods has led to substantial adoption of this technique for treatment of limbal stem cell deficiency. The mRNA expression profiles of AMLE and CE were assayed using microarrays. Transcripts with a 1.5-fold change in either direction in addition to a Bonferroni adjusted P value < 0.05 were considered to be differentially expressed. Expression changes detected by microarray profiling and important corneal-limbal markers were assessed using quantitative real-time PCR (qRT-PCR) and immunofluorescence staining. A total of 487 probe sets (319 upregulated and 168 downregulated) were found to be differentially expressed between AMLE and CE. Enrichment analysis revealed significant overrepresentation of multiple biological processes (e.g., response to wounding, wound healing, and regulation of cell morphogenesis) within the differentially expressed gene list. The expression of a number of genes that were upregulated (ABCG2, S100A9, ITGA5, TIMP2, FGF5, PDGFC, SEMA3A) and downregulated (KLF4, P63α) in AMLE was confirmed using qRT-PCR. Immunofluorescence confirmed that AMLE cultures were P63α, ABCG2, CK3, CK12, and E-cadherin (E-cad) positive. In this study, we have shown that genes associated with wound healing processes are upregulated in AMLE. These gene expression changes may contribute to corneal restoration and the positive outcomes associated with transplantation.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Finbarr O'Sullivan, William Power, Martin Clynes, Conor Murphy, Andra Bobart-Hone, Sandra Shaw, Vincent Lynch, Padraig Doolan, Kishore R Katikireddy, Sinéad T Aherne and 2 others

Experts in our system

1
Finbarr O'Sullivan
Dublin City University
Total Publications: 21
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Vincent Lynch
Dublin City University
Total Publications: 10
 
4
Padraig Doolan
Dublin City University
Total Publications: 37
 
5
Sinéad T Aherne
Dublin City University
Total Publications: 17