Type

Journal Article

Authors

Paula Meleady
Annemarie Larkin
Martin Clynes
Michael Henry
Shane Kelly
Gustavo B Baretton
Katrin Friedrich
Priyanka Maurya
Annett Linge

Subjects

Microbiology

Topics
mortality humans tandem mass spectrometry metabolism dna binding proteins carcinoma ductal breast female prevention control disease progression rad23b protein human pathology dna repair enzymes kaplan meier estimate cell nucleus cell line tumor breast neoplasms cytoplasm receptors estrogen

Identification and functional validation of RAD23B as a potential protein in human breast cancer progression. (2014)

Abstract Identification of protein targets that play a role in breast cancer invasion may help to understand the rapid progression of cancer and may lead to the development of new biomarkers for the disease. In this study, we compared two highly invasive and two poorly invasive breast cancer cell lines using comparative label-free LC-MS profiling in order to identify differentially expressed proteins that may be linked to the invasive phenotype in vitro. Forty-five proteins were found to be upregulated, and 34 proteins, downregulated. UV excision repair protein RAD23 homologue B (RAD23B) was found among the downregulated proteins in highly invasive breast cancer cell lines. In poorly invasive breast cancer cell lines, siRNA-mediated downregulation of RAD23B subsequently led to an increase in invasion and adhesion in vitro. Immunohistochemistry analysis of 164 specimens of invasive breast cancer showed that having a high percentage (>80%) of RAD23B positive nuclei was significantly associated with histopathological grades 1 and 2 breast cancer and with low mitotic activity. In addition, a high staining intensity for RAD23B in the cytoplasm was significantly associated with histopathological grade 3 breast cancer. This study suggests a potential role of RAD23B in breast cancer progression and may further imply a tumor suppressor role of nuclear RAD23B in breast cancer.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Paula Meleady, Annemarie Larkin, Martin Clynes, Michael Henry, Shane Kelly, Gustavo B Baretton, Katrin Friedrich, Priyanka Maurya, Annett Linge

Experts in our system

1
Paula Meleady
Dublin City University
Total Publications: 95
 
2
Annemarie Larkin
Dublin City University
Total Publications: 25
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Michael Henry
Dublin City University
Total Publications: 78
 
5
Shane Kelly
Dublin City University
Total Publications: 6