Type

Journal Article

Authors

Jens Ducree
Gregor Kijanka
David J. Kinahan
Danielle Chung
Daniel Kirby
Macdara Glynn

Subjects

Medicine & Nursing

Topics
blood cells future magnetic field microfluidic chip hiv aids point of care biological samples medical diagnostics

Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications. (2013)

Abstract In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation of (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels of expertise, expense, and multistep protocols. Increasing automation, miniaturization, and availability of such technologies is an aim of microfluidic lab-on-a-chip strategies. To this end, we present an integrated, dual-force cellular separation strategy using centrifugo-magnetophoresis. Whole blood spiked with target cells is incubated with (super-)paramagnetic microparticles that specifically bind phenotypic markers on target cells. Under rotation, all cells sediment into a chamber located opposite a co-rotating magnet. Unbound cells follow the radial vector, but under the additional attraction of the lateral magnetic field, bead-bound target cells are deflected to a designated reservoir. This multiforce separation is continuous and low loss. We demonstrate separation efficiently up to 92% for cells expressing the HIV/AIDS relevant epitope (CD4) from whole blood. Such highly selective separation systems may be deployed for accurate diagnostic cell isolations from biological samples such as blood. Furthermore, this high efficiency is delivered in a cheap and simple device, thus making it an attractive option for future deployment in resource-limited settings.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Jens Ducree, Gregor Kijanka, David J. Kinahan, Danielle Chung, Daniel Kirby, Macdara Glynn

Experts in our system

1
Jens Ducree
Dublin City University
Total Publications: 93
 
2
Gregor Kijanka
Dublin City University
 
3
David J Kinahan
Dublin City University
Total Publications: 47
 
4
Danielle Chung
Dublin City University
Total Publications: 6
 
5
Daniel Kirby
Dublin City University
Total Publications: 6
 
6
Macdara Glynn
Dublin City University
Total Publications: 22