Type

Journal Article

Authors

Niall Barron
Martin Clynes
Paula Meleady
Alfonso Blanco
Sinead Aherne
Padraig Doolan
Colin Clarke
Clair Gallagher
Nga Lao
Mark Gallagher
and 1 others

Subjects

Biochemistry

Topics
up regulation down regulation cho cells cell survival dna repair phase transition cell growth cell cycle arrest

MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. (2012)

Abstract MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Niall Barron, Martin Clynes, Paula Meleady, Alfonso Blanco, Sinead Aherne, Padraig Doolan, Colin Clarke, Clair Gallagher, Nga Lao, Mark Gallagher and 1 others

Experts in our system

1
Niall Barron
Dublin City University
Total Publications: 44
 
2
Martin Clynes
Dublin City University
Total Publications: 209
 
3
Paula Meleady
Dublin City University
Total Publications: 95
 
4
Sinéad T Aherne
Dublin City University
Total Publications: 17
 
5
Padraig Doolan
Dublin City University
Total Publications: 37
 
6
Colin Clarke
Dublin City University
Total Publications: 26
 
7
Clair Gallagher
Dublin City University
Total Publications: 12
 
8
Nga T Lao
Dublin City University
Total Publications: 7
 
9
Mark Gallagher
Dublin City University
Total Publications: 4