Type

Journal Article

Authors

Jens Ducrée
Richard O'Kennedy
Colette McDonagh
Stephen Daniels
Louise Barrett
Martha Rook
Christopher Martin
Julie Murrell
Stephen Hearty
Robert Burger
and 8 others

Subjects

Physics

Topics
lab on a disc rapid detection immunoglobulin g line flow control serial siphon integrated optics plasma enhanced chemical vapour deposition

At-line bioprocess monitoring by immunoassay with rotationally controlled serial siphoning and integrated supercritical angle fluorescence optics. (2012)

Abstract In this paper we report a centrifugal microfluidic "lab-on-a-disc" system for at-line monitoring of human immunoglobulin G (hIgG) in a typical bioprocess environment. The novelty of this device is the combination of a heterogeneous sandwich immunoassay on a serial siphon-enabled microfluidic disc with automated sequential reagent delivery and surface-confined supercritical angle fluorescence (SAF)-based detection. The device, which is compact, easy-to-use and inexpensive, enables rapid detection of hIgG from a bioprocess sample. This was achieved with, an injection moulded SAF lens that was functionalized with aminopropyltriethoxysilane (APTES) using plasma enhanced chemical vapour deposition (PECVD) for the immobilization of protein A, and a hybrid integration with a microfluidic disc substrate. Advanced flow control, including the time-sequenced release of on-board liquid reagents, was implemented by serial siphoning with ancillary capillary stops. The concentration of surfactant in each assay reagent was optimized to ensure proper functioning of the siphon-based flow control. The entire automated microfluidic assay process is completed in less than 30 min. The developed prototype system was used to accurately measure industrial bioprocess samples that contained 10 mg mL(-1) of hIgG.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Jens Ducrée, Richard O'Kennedy, Colette McDonagh, Stephen Daniels, Louise Barrett, Martha Rook, Christopher Martin, Julie Murrell, Stephen Hearty, Robert Burger and 8 others

Experts in our system

1
Jens Ducrée
Dublin City University
Total Publications: 33
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Colette McDonagh
Dublin City University
Total Publications: 47
 
4
Stephen Daniels
Dublin City University
Total Publications: 64
 
5
Stephen Hearty
Dublin City University
Total Publications: 27
 
6
Robert Burger
Dublin City University
Total Publications: 18