Type

Journal Article

Authors

Niall Barron
Sinéad T Aherne
Martin Clynes
Lin Zhang
Mark Leonard
Paula Meleady
Laura Breen
Paula Kinsella
Colin Clarke
Padraig Doolan

Subjects

Biochemistry

Topics
growth development cell cycle cell line cell growth growth rate cell lines in silico analysis gene expression

Transcriptomic analysis of clonal growth rate variation during CHO cell line development. (2012)

Abstract The selection of clones displaying a high rate of cell growth is an essential component of Chinese hamster ovary (CHO) cell line development. In recent years various "omics" technologies have been utilised to understand the mechanisms underlying bioprocess phenotypes. In this study, gene expression analysis using a CHO-specific microarray was conducted for a panel of CHO-K1 MAb-secreting cell lines spanning a range of growth rates that were derived from a single cell line development project. In-silico functional analysis of the resulting transcriptomic data revealed the overrepresentation of biological processes such as cell cycle and translation within those genes upregulated during fast growth, while genes associated with cellular homeostasis were downregulated. Using differential expression and correlation analysis we identified a high priority group of 416 transcripts (190 upregulated; 226 downregulated) associated with growth rate. Expression changes of eight of these genes were independently confirmed by qPCR. Finally, we demonstrate the enrichment of predicted mRNA targets of miR17-92, a microRNA (miRNA) cluster known to be upregulated during rapid proliferation, within downregulated transcripts.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Niall Barron, Sinéad T Aherne, Martin Clynes, Lin Zhang, Mark Leonard, Paula Meleady, Laura Breen, Paula Kinsella, Colin Clarke, Padraig Doolan

Experts in our system

1
Niall Barron
Dublin City University
Total Publications: 44
 
2
Sinéad T Aherne
Dublin City University
Total Publications: 17
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Paula Meleady
Dublin City University
Total Publications: 95
 
5
Laura Breen
Dublin City University
Total Publications: 11
 
6
Colin Clarke
Dublin City University
Total Publications: 26
 
7
Padraig Doolan
Dublin City University
Total Publications: 37