Type

Journal Article

Authors

Richard O'Kennedy
Paul Leonard
Stephen Hearty

Subjects

Biochemistry

Topics
immunology antigen antibody reactions immobilized proteins instrumentation surface plasmon resonance reference standards buffers metabolism kinetics

Measuring antibody-antigen binding kinetics using surface plasmon resonance. (2012)

Abstract Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a diverse range of protein-protein interactions and is considered almost de rigueur for characterizing antibody-antigen interactions. The technique obviates the need to label either of the interacting species and the binding event is visualized in real-time. Thus, it is ideally suited for screening crude, unpurified antibody samples that dominate early candidate panels following antibody selection campaigns. SPR returns both concentration and affinity data but when used correctly can also resolve the discrete component kinetic parameters (association and dissociation rate constants) of the affinity interaction. Herein, we outline some SPR-based generic antibody screening configurations and methodologies in the context of expediting data-rich ranking of candidate antibody panels and ensuring that antibodies with the optimal kinetic binding characteristics are reliably identified.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Richard O'Kennedy, Paul Leonard, Stephen Hearty

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Paul Leonard
Dublin City University
Total Publications: 27
 
3
Stephen Hearty
Dublin City University
Total Publications: 27