Type

Journal Article

Authors

Robert J. Forster
Tia E. Keyes
Hazel McArdle
Eoin Brennan
Elaine Spain

Subjects

Microbiology

Topics
metal nanoparticles polymerase chain reaction nucleic acid hybridization dna bacterial electrochemical techniques staphylococcus aureus oxidation reduction gold platinum chemistry genetics catalysis stereoisomerism electrodes hydrogen peroxide

High sensitivity DNA detection based on regioselectively decorated electrocatalytic nanoparticles. (2012)

Abstract Self-assembled monolayers (SAMs) of dodecanethiol have been formed on gold electrodes to produce nanoscale defects. These defects define nucleation sites for the electrodeposition of mushroom shaped platinum nanoparticles (PtNPs). The top surfaces of these PtNPs have been selectively functionalized with single stranded probe DNA. These regioselectively modified particles were desorbed by applying a current jump to yield nanoparticles capable of biorecognition on the top curved side and efficient electrocatalysis on the nonfunctionalized lower surface. A second electrode was functionalized with single stranded capture DNA that has a sequence that is complementary to the pathogen, Staphylococcus aureus but leaves a section of the target available to bind the probe strand immobilized on the PtNPs. Following hybridization of the target and capture strands, the surface was exposed to the probe DNA labeled electrocatalytic PtNPs. Target binding was detected by monitoring the current associated with the reduction of hydrogen peroxide in a solution of 0.01 M H(2)SO(4). Calibration plots of the log[DNA] versus faradaic current were linear from 10 pM to 1 μM and picomolar concentrations could be detected without the need for amplification of the target, for example, using PCR or NASBA. As well as a wide dynamic range, this detection strategy has an excellent ability to discriminate DNA mismatches and a high analytical sensitivity.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Robert J. Forster, Tia E. Keyes, Hazel McArdle, Eoin Brennan, Elaine Spain

Experts in our system

1
Robert J. Forster
Dublin City University
Total Publications: 92
 
2
Tia E. Keyes
Dublin City University
Total Publications: 104
 
3
Hazel McArdle
Dublin City University
Total Publications: 6
 
4
Eoin P Brennan
University College Dublin
Total Publications: 18
 
5
Elaine Spain
Dublin City University
Total Publications: 15